Extensive biodegradation of Nigerian Crude Oil (Escravos Light) by newly Characterized Yeast Strains.
No Thumbnail Available
Date
2011
Authors
Ilori, M.O
Adebusoye, S.A.
Obayori, S.O.
Oyetibo, G.O.
Ajidahun, O.
James, C.
Amund, O.O
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Because microbial degradation is known to be an efficient process in the in situ decontamination of
oil-bearing environments, it is believed that development of effective bioremediation strategies will
be aided by microbial sourcing of novel and competent hydrocarbon degraders with a broad and
unusual substrate spectrum. Thus, in keeping with this objective, two Candida strains (MNI and
MCI) isolated after a repeated batch enrichment technique were tested for their biodegradation
potentials on Nigerian crude oil, Escravos light. Axenic cultures of strains MNI and MCI grew at a
rate of 1.623 and 0.586 d-1, respectively, in mineral salts medium supplemented with 8.4 g L-1 of
crude oil. Whereas strain MNI degraded aliphatic fractions by 97.6% and the aromatics by 74.61%,
the corresponding values obtained for MCI were 97.2% and 67.29% during the 14-day incubation
period. The gas chromatography (GC) fingerprinting of aliphatic fractions showed major degradation
of heptadecane (C17), octadecane (C18), nonadecane (C19), eicosane (C20), undodecane (C21),
tricosane (C23), hexacosane (C26), octacosane (C28), and nonacosane (C29) in less than 6 days,
whereas nearly 100% of these fractions including the isoprenoid molecules was metabolized in 14
days. Among the aromatic fractions that were nearly eliminated during the cultivation period were
naphthalene, phenanthrene, fluoranthrene, chrysene, benzo(a)anthracene, benzo(b)fluoranthrene, and
benzo(a)pyrene. Intrestingly, substrate uptake studies showed that both strains grew very well on
petroleum cuts, biphenyl, phenol, xylene, and quite a number of polycyclic aromatic hydrocarbons
including pyrene, phenanthrene, and anthracene.
Description
To access the full text of this paper, kindly send email to the repository administrators: Dr (Mrs) Yetunde Zaid (yzaid@unilag.edu.ng) and Dr Christopher Okiki (cokiki@unilag.edu.ng
Keywords
Bioremediation , Hydrocarbon , Polycyclic aromatic hydrocarbons , Aromatic fractions
Citation
Ilori, M.O., Adebusoye, S.A., Obayori, S.O., Oyetibo, G.O., Ajidahun, O., James, C. and Amund, O.O. (2011). Extensive biodegradation of Nigerian Crude Oil (Escravos Light) by newly Characterized Yeast Strains. Petroleum Science and Technology 29: 2191-2208.