Biodegradation of crude oil and phenanthrene by heavy metal resistant Bacillus subtilis isolated from a multi-polluted industrial wastewater creek
No Thumbnail Available
Date
2017-02
Authors
Oyetibo, G.O
Chien, M.F
keda-Ohtsubo, W
Suzuk, H
Obayori, O.S
Adebusoye, S.A
Ilori, M.O
Amund, O.O
Endo, O
Journal Title
Journal ISSN
Volume Title
Publisher
International Biodeterioration & Biodegradation (ELSEVIER)
Abstract
A critical bottleneck associated with bioremediation technology in multi-polluted environments is
microbiostasis due to metal toxicity. Autochthonous Bacillus species that would harness a repertory of
traits to catabolize hydrocarbons and simultaneously sequester heavy metals (HMs) is invaluable in the
environment contaminated with divergent pollutants. Fourteen HM-resistant bacilli from polluted creek
were characterized using phenotypic and molecular criteria, and studied for hydrocarbon degradation in
chemically defined media amended with Co2þ and Ni2þ (5.0 mmol l 1 each). Phylogenetic analyses
revealed distribution of the bacilli into three clades. Two dissimilar strains of Bacillus subtilis (M16K, and
M19F) with 19.1% sequence divergence, exhibited excellent degradation of crude oil (>94.0%) with evidence
of early degradation of isoprenoid hydrocarbons and concurrent metal removal 18 d postinoculation.
Similarly, phenanthrene degradation (>85.0%), and corresponding metal detoxification
occurred in 28 d axenic culture of the strains. Strain M16K and M19F were metabolically active in
matrices containing HMs, degraded hydrocarbons and simultaneously removed HMs from the medium.
To the best of our knowledge, this is the first report of metal-resistant Bacillus subtilis strains showing
simultaneous degradation of hydrocarbons and detoxification of metals, particularly in the Sub-Saharan
Africa. The bacilli could be useful as potential biological agents in effective bioremediation campaign for
multi-polluted environments.
Description
For full text click on http://www.sciencedirect.com/science/article/pii/S0964830517302494
Keywords
Heavy metals , Crude oil , Phenanthrene , Heavy metal-resistant , Biodegradation , Metal-biosequestration
Citation
Oyetibo, G. O., Chien, M. F., Ikeda-Ohtsubo, W., Suzuki, H., Obayori, O. S., Adebusoye, S. A.,M. Oi Ilori, O. O. Amund & Endo, G. (2017). Biodegradation of crude oil and phenanthrene by heavy metal resistant Bacillus subtilis isolated from a multi-polluted industrial wastewater creek. International Biodeterioration & Biodegradation, 120, 143-151.