Recent Submissions

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Open Access
INTERPERITONEAL VS ORAL ROUTE OF ALCOHOL ADMINISTRATION,WHICH IS BETTER?
(Global Research Journal of Microbiology, 2013) Ebuehi, O.A.T.; Dawodu, O.G.; Odesanmi, O.S.; Buari, O.S.
The major problem researchers of alcohol intoxication and its dependence face is the route of administration as animals though good do not mimic the perfect subjects i.e. human beings. This study however compares between 2 accepted means of alcohol intoxication namely interperitoneal (i.p) and oral (o.r) routes. A total of 24 Rats were divided into 4 groups vis-à-vis (a) oral group administered with 63mg (10%) and 130mg (20%) ethanol kg-1 body weight (chronic) for a period of 14 days and concomitantly, the intraperitoneal group, injected with 130mg (20%) ethanol kg-1 body weight for a period of 7 days (acute) and lastly the control group injected with normal saline as the body weight dictated. After this period of intoxication the following biochemical parameters were determined ALT, AST, Cholesterol and Electrolyte balance. Ethanol intoxicated rats (chronic and acute) recorded a significant increase in serum aspartate (AST) and Alanine (ALT) amino transferase activities, cholesterol, and a decrease in electrolyte (potassium) the elevation in total protein was not significant with the i.p group showing the highest elevations. Hence, aspartate (AST) and alanine (ALT) aminotransferase, cholesterol, electrolyte estimation (potassium) may be used as biomarkers for the early diagnosis of ethanol toxicity in human beings.
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Open Access
Ebuehi, O.A.T.,Erukainure, O.L.,Adeboyejo, F.O.,Elemo, G.N. Modulation of lipid peroxidation, hypolipidemic and antioxidant activities in brain tissues of diabetic rats by fibre - Enriched biscuits
(Journal of Acute Disease, 2012) Ebuehi, O.A.T.; Erukainure, O.L.; Adeboyejo, F.O.; Elemo, G.N.
Objective: To investigate the effect of feeding fibre - enriched biscuit on the antioxidant and hypolipidemic activities in brain tissues of diabetic rats. Method: Diabetes was induced by a single intraperitoneal injection of alloxan. Treatment lasted for 14 d, after which the rats were sacrificed by cervical dislocation. Brain tissues were used for the assessment of GSH, catalase, SOD and lipid peroxidation as well as lipid profiles. Result: Induction of diabetes led to a significant decrease in GSH level, elevated SOD and catalase activities. These were significantly modified by the biscuits. There was an elevated level of malondialdehyde in the brain tissues of the untreated diabetic rats; this was significantly reduced by the biscuits. There was a significant decrease in HDL and a significant increase in LDL levels, total cholesterol and triglycerides in the untreated (diabetic) rats. Feeding with fibre - enriched biscuits led to decrease in the levels of total cholesterol, triglyceride, LDL - cholesterol and caused a significant increase in the levels of HDL. Conclusions: These results suggest a therapeutic and protective effect of the fibre - enriched biscuits against diabetic - induced brain toxicity in rats.
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Open Access
Building a Successor-Generation: Reflections on Values and Knowledge in Nation Building
(2017-01-23) Fayemi, K.
It is with great pride that I participate in the 2017 Convocation ceremony of my alma mater - the University of Lagos - UNILAG. It is always fascinating to return to these scenic and historic grounds of learning, situated in one of the most dynamic cities in the world. I am most grateful to the authorities for honouring me with this hallowed platform to share a few thoughts with this set of graduating students in particular and the university community at large as this year's convocation lecturer. When the Vice Chancellor, Prof. Rahamon A. Bello first notified me of my being selected to deliver the 2015/2016 convocation lecture, the first thought that came to my mind was "what would I say?" Convocation lectures traditionally have a defined structure. The university authorities usually invite an individual that has attained some measure of success in life, to share hislher story, with the hope that the graduating students can learn a thing or two about what to expect in the 'real world', as the larger society is often called. So I assume I am expected to speak about my experiences in life, in the more than three decades since I graduated, with the hope that those whose turn it is today can have an idea of at to expect out there. I am also supposed to dispe se some hope about the bright prospects that the olds, and offer some useful advice that would guide e going forward. Considering these expectations of the . ional convocation speech, I must apologise in advance eradventure I don't live up fully to this billing. This is because my presentation today would dwell more on pointing you back to some of life's key lessons that are available within a university, which are enough to prepare you for life after graduation. Many years ago, I matriculated into this university, thrown into a whole new universe, not knowing what to expect. As a na'ive and unassuming youth, all I came to UNILAG with was eagerness to learn and zeal to explore. At graduation, I was undoubtedly a different person. This institution provided the conditions for me to discover myself; find my voice; and hone my worldview and core values
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Open Access
Modulation of lipid peroxidation, hypolipidemic and antioxidant activities in brain tissues of diabetic rats by fibre - Enriched biscuits
(Journal of Acute Disease, 2012) Ebuehi, O.A.T.; Erukainure, O.L.; Adeboyejo, F.O.; Elemo, G.N.
Objective: To investigate the effect of feeding fibre - enriched biscuit on the antioxidant and hypolipidemic activities in brain tissues of diabetic rats. Method: Diabetes was induced by a single intraperitoneal injection of alloxan. Treatment lasted for 14 d, after which the rats were sacrificed by cervical dislocation. Brain tissues were used for the assessment of GSH, catalase, SOD and lipid peroxidation as well as lipid profiles. Result: Induction of diabetes led to a significant decrease in GSH level, elevated SOD and catalase activities. These were significantly modified by the biscuits. There was an elevated level of malondialdehyde in the brain tissues of the untreated diabetic rats; this was significantly reduced by the biscuits. There was a significant decrease in HDL and a significant increase in LDL levels, total cholesterol and triglycerides in the untreated (diabetic) rats. Feeding with fibre - enriched biscuits led to decrease in the levels of total cholesterol, triglyceride, LDL - cholesterol and caused a significant increase in the levels of HDL. Conclusions: These results suggest a therapeutic and protective effect of the fibre - enriched biscuits against diabetic - induced brain toxicity in rats.
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Open Access
Antiproliferative Potential of Ethanol Leaf Extract of Motandra guineensis (Thonn.) A.DC. (Apocynaceae) against Human Melanoma and Ovarian Cancer Cells
(Natural Products Research Group, University of Benin, 2024-04-01) Oiseoghaede, J.O.; Oyawaluja, A.A.; Sowemimo, A.A.; Odukoya, O.A.; Che, C-T.
In ethnomedicine, Motandra guineensis (Thonn.) A.DC. (Apocynaceae) is used to massage abscessed gums and manage pain. This study aimed to evaluate the antiproliferative effect of leaves of M. guineensis against human ovarian (OVCAR3) and melanoma (MDA-MB-435) skin cancer cells. Crude ethanol extract and fractions of M. guineensis leaves were evaluated for antiproliferative effect on OVCAR3 and MDA-MB-435 cells using cell viability assay at 2 and 20 µg/ml. In addition, the inhibitory effect of extract and fractions on nitric oxide (NO) production with lipopolysaccharide (LPS) stimulation (Greiss assay) on murine macrophage cells (RAW 264.7) at 0.3125 – 10 µg/ml was evaluated. Cell viability was evaluated using murine macrophage cells while toxicity assessment was done on Vero cells. Antiproliferative activity profile (% cell death) of extract and fractions followed the order: butanol (30% and 23%) > aqueous (28% and 21%) > ethyl acetate (22% and 19%) > crude (22% and 0%) at 20 μg/ml in OVCAR3 and MDA MB-435 cell lines respectively. Percentage NO inhibitory activity showed hexane fraction with the highest activity and aqueous fraction with the least activity at 10 μg/ml. Extract and fractions were less toxic in Vero cells when compared to the standard drug, Tamoxifen. Toxicity assessment using murine macrophage cells showed no significant difference in cell viability when compared to standard. Results indicate antiproliferative potential, NO inhibitory potential and safety of crude ethanol leaf extract and fractions of M. guineensis.