The Nutritional (Carbon & Nitrogen) Requirements for Growth of Four Fungal Isolates Associated with Soft Rot of Yams in Storage in Nigeria.
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The effects of some carbon and nitrogen compounds and of some fungicides on the growth of Botryodiplodia theobromae Pat, Fusarium moniliforme We & Rg, Aspergilus niger Van Tiegh and Apergilus parasiticus Speare, were studied. When the carbon compounds were incorporated into Czapek Dox medium at 12.62 gm C/litter, glucose, maltose sucrose, and fructose were generally better utilised than mannitoo, galactose and lactose. However, maximal mycelial production of F. moniliforme was obtained with mannitol. Dry weights of mycelia of all the fungi used generally increased with increase in carbon concentration but the carbon utilisation factor (mg dry w. mycelium x 100) mg carbon/litre decrease correspondingly. Of the four nitrogen sources used at a concentration of 0.494 gm N/litre, sodium nitrate was the best for the growth of B. theobromae. F. moniliforme, A. niger and A. parasiticus had maximal mycelial yield with glutamine as N-source. Nitrogen utilisation factor decreased with increase in Nitrogen concentration. of the three fungicides used, captan (N-trichloromethylthio -4- cyclohexane-1, 2-dicarboximide) used at 100, 80, 60, 40, and 20 ppm did not inhibit growth of the four fungi while benlate (1-butylcarbamoyl-2-benzimidazole carbamic acid, methyl ester) at 80, 60, 40 and 20 ppm an thiabendazole (2,4-thiazolyl benzimidazole) at the above concentrations completely inhibited growth of B. theobromae and to a lesser extent growth of A. niger, F. moniliforme and A. parasiticus.