Volume 3, Issue 2, 2015

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Browsing Volume 3, Issue 2, 2015 by Author "Ogwu, M.C"

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  • Item
    Open Access
    Comparative Micro-anatomical Studies of the Wood of two Species of Okra [Abelmoschus Species]
    (University of Lagos Press, Akoka, 2016) Osawaru, M.E; Aiwansoba, R.O; Ogwu, M.C
    Okra belongs to the family Malvaceae. Common edible species are either Abelmoschus caillei [A. Chev.] Stevels or A. esculentus Moench. Seeds of the two species were obtained from the Gene bank of National Centre for Genetic Resources and Biotechnology, Ibadan, Nigeria. This study anatomically investigated the accessions to determine their distinctiveness and assess their level of diversity. Field trials were conducted at the University of Benin, Nigeria. The main stem from tagged point at seven weeks interval at three points were investigated from three dimensional views (transverse, radial and tangential section). Using light microscopy, the nature and composition of the wood were determined from the macerated part. Twenty random fibers were measured from each representative sample slide. The occurrence of the growth rings were consistent in both species showing ring porous arrangements. The vessels in A. esculentus were solitary and short radial multiples in arrangement and A. caillei were short radial multiples and irregular clusters in arrangement but both species had mainly simple perforation vessels. More so, the distribution of axial parenchyma was of paratracheal orientation. A. caillei had wide and high multiseriate rays while in A. esculentus only high multiseriate rays were observed. There was a reduction in vessel diameter and fiber length across the age in both species. Fiber diameter, fiber lumen and fiber cell wall showed different degree of fluctuations with age in both species. The study suggests that age may significantly influence anatomical characters of both species.
  • Item
    Open Access
    Rapd Pcr Genotyping of Ten Accessions of Tomato (Lycopersicon esculentum Con. [Mill.] Syn. Solanum lycopersicon [Linn.])
    (University of Lagos Press, Akoka, 2015) Ogwu, M.C; Chime, A.O; Osawaru, M.E; Aiwansoba, R.O; Gbadebo, F
    Tomato (Lycopersicon esculentum Con. [Mill.] Syn. Solanum lycopersicon [Linn.]) belongs to the family Solanaceae and order Solanales. The plant originated from South America but has been domesticated worldwide. This study investigated the variation in Tomato germplasm using RAPD PCR genotyping technique. Seven accessions; NGB01254, NGB01250, NGB01302, NG/DE/MAR/09/019, NGB01301, NGB01255 and NGB 01237 were collected from Gene Bank while three accessions were obtained from home gardens including DE/IK-L-001, DE/IK-L-002 and ED/EG-L-001. The samples were cultivated in a Screen House in the University of Benin, Benin City, Nigeria. Standard procedures were used for DNA extraction and purification while the commercial primer OPA-09 (51–GGG TAA CGC C-31) was employed for genotyping. Each DNA fragment generated from RAPD PCR genotyping was treated as a separate character and scored as a discrete variable, which was used to distinguish the accessions. Results suggest the primer was able to amplify less than 50 % of the samples studied but was able to amplify more than 50 % of the collections from home gardens. Three genetic types were observed, which may be related to nutrients, growth condition and species of Tomatoes studied. Of the ten samples studied, 4 (40.0 %) were amplified and typeable with the OPA 09 commercial primer while the remaining 6 (60.0 %) were not typeable with this primer. Although this study have further enumerated the advantages of molecular markers in plant characterization, it is important that a combination of molecular and morphological traits be considered for effective characterization of plant germplasm.