Growth of Pseudomonas aeruginosa LP 5 on 2,5-Dichlorobenzoate: Detection of Aromatic Ring Hydroxylating Dioxygenase Gene
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Date
2011
Authors
Obayori, S.O
Ilori, M.O
Amund, O.O
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Abstract
Pseudomonas aeruginosa LP5 grew on 2, 5-dichlorobenzoate with doubling time (D) 6.64 d and mean
growth rate (k) 0.104 d-1. The organism showed a prolonged lag period lasting 9 days followed by a
sudden rise within 3 days (D=1.1 d; k =0.628 d-1) and death in less than 72 hours on 2,6-
dichlorobenzoate. Polymerase chain reaction (PCR) amplification of DNA of LP5 showed aromatic
dihydroxylating (ARHDO) gene band with molecular weight corresponding to the targeted fragment
(0.73 kb). The capability of LP5 on dichlorobenzoates and detection of dioxygenase genes is a
validation of its versatility and potential for bioremediation.
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Keywords
Dichlorobenzoates , Bioremediation , Aromatic dihydroxylating , Dioxygenase genes
Citation
Growth of Pseudomonas aeruginosa LP 5 on 2,5-Dichlorobenzoate: Detection of Aromatic Ring Hydroxylating Dioxygenase (ARHDO) Gene. International Research Journal of Microbiology 2(2): 085-089.