Biodegradation of Selected Polycyclic Aromatic Hydrocarbons by Axenic Bacterial Species Belonging to the Genera Lysinibacillus and Paenibacillus
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Date
2017-08
Authors
Nwinyi, O.C
Amund, O.O
Journal Title
Journal ISSN
Volume Title
Publisher
Springer International Publishing
Abstract
The quest for competent degraders of recalcitrant polynuclear aromatic hydrocarbons (PAHs) for use in sustainable bioremediation technology has justified the execution of this work. In this study, three bacterial strains (FB-1, FB-2 and FB-3) were isolated from former industrial site in Bloomington, Indiana. The catabolic versatility of these obtained strains were evaluated on some selected PAH- naphthalene, anthracene, fluoranthene and pyrene. Using the 16S r RNA sequencing analyses, our strains belonged to the family Firmicutes wereby, strain FB-1 was identified as Lysinibacillus sp. FB-1, strain FB-2 as Bacterium FB-2 and strain FB-3 as Lysinibacillus fusiformis FB-3. The biodegradation of the selected PAHs was determined using gas chromatography and the calculated percentage utilization of the selected PAHs varied between 97 to 4%. We further determined the mean biodegradation rates for fluoranthene when supplemented with molasses. The mean biodegradation rates were between (mgL-1) 0.214± 0.006 -0.318 ± 0.002, while MS-fluoranthene only ranged between (mgL-1) 0.210 ± 0.056 - 0.437± 0.176. However, with ANOVA at 5% (P< 0.05) there seemed to be no significant difference in the mean biodegradation rates between both media. These findings may have practical and ecological prospects in designing and improving bioremediation of polycyclic aromatic hydrocarbon contaminated sites..
Description
Staff Publications
Keywords
Competent Degraders , Bioremediation , Molasses
Citation
Nwinyi, O.C and Amund, O.O (2017) Biodegradation of Selected Polycyclic Aromatic Hydrocarbons by Axenic Bacterial Species Belonging to the Genera Lysinibacillus and Paenibacillus.Iranian Journal of Science and Technology, Vol.39