Investigation of Chemical, Genotoxic and Haematological Properties of Secondary Metabolites from n-Hexane Extract of Olax subscorpioidea Oliv. (Olacaceae) Leaves
No Thumbnail Available
Date
2018
Authors
Oladipupo, A.R.
Alaribe, S.C.
Olalekan, B.
Akere, H.
Ayanda, F.
Emuejevoke, T.
Coker, H.A
Journal Title
Journal ISSN
Volume Title
Publisher
Tropical Journal of Natural Product
Abstract
Due to their phytochemical diversity, plants may exhibit various biological activities. They may
be medicinal as well as toxic. As a result, investigating their toxicities is as important as
investigating their medicinal properties. This study investigated the chemical, genotoxic and
haematological properties of the n-hexane extract of Olax subscorpioidea leaves. The chemical
investigation of the extract was done by gas chromatography-mass spectrometry (GC-MS)
analysis. Genotoxic and haematological investigation were done using in vivo models in mice.
Genotoxic effects were estimated by evaluating DNA fragmentation with agarose gel
electrophoresis and diphenylamine (DPA) assays. Haematological indices were measured on an
Auto Haematology Analyzer. With GC-MS analysis, eight compounds were identified in the
extract. The major compounds were 9,12-octadecadienoic acid (Z, Z)- (18.00%), n-hexadecanoic
acid (17.60%), and squalene (4.12%), which are of reported medicinal properties. Genotoxicity
results indicated that the extract markedly and significantly reduced DNA fragmentation in
testicular and hepatic cells in mice. Haematological analysis showed that the extract significantly
increased white blood cells (WBC), lymphocytes (LYMP), mean corpuscular volume (MCV),
mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration
(MCHC), and significantly reduced packed cell volume (PCV), haemoglobin concentration (HB),
and red blood cell (RBC) count. These results indicate that the n-hexane extract of Olax
subscorpioidea leaves contain compounds that possess useful pharmacologic properties, and may
reduce DNA damages as well as alter haematological indices.
Description
Scholarly article
Keywords
Toxicity , GC-MS , phytochemicals , genotoxicity , DNA , haematological indices , Research Subject Categories::PHARMACY
Citation
Oladipupo AR, Alaribe CS, Balogun OT, Akere HT, Ayanda FA, Toye ET, Coker HAB. Investigation of Chemical, Genotoxic and Haematological Properties of Secondary Metabolites from n-Hexane Extract of Olax subscorpioidea Oliv. (Olacaceae) Leaves. Trop J Nat Prod Res. 2018; 2(12):506-511. https://doi.org//10.26538/tjnpr/v2i12.3