Histomorphological response of sulforaphane and riboceine on annular puncture-induced model of rabbits intervertebral disc degeneration
Loading...
Date
2017
Authors
Ogunlade, B
Osinubi, A.A
Duru, F.I.O
Adelakun, S.A
Ogunlade, S.A
Alao, A.A
Journal Title
Journal ISSN
Volume Title
Publisher
European Journal of Biomedical and Pharmaceutical Sciences
Abstract
Background: Intervertebral disc degeneration is one of the leading causes of Low back pain that is regarded as
one of the most frequent neurological ailments. Objective: To determine the regenerative potential of
sulforaphane and riboceine supplements in a rabbit model in annular punctured intervertebral disc degeneration.
Methods: Forty New Zealand white rabbits (3 to 3.5 kg each) underwent annular puncture of the L2-L3, L3-L4,
and L4-L5 discs. Rabbits were sacrificed at 2, 4 and 8 after puncture. For a longitudinal study to assess changes in
disc height over time, serial X-rays were performed at 0, 4 and 8 weeks for the rabbits. Upon sacrifice, the whole
spinal column and discs were extracted and analyzed with real time reverse transcriptase-polymerase chain
reaction, and histological staining. Results: The radiology assessment revealeda significant progressive decrease
in disc height which was reversed by the administration of sulforaphane and riboceine. Significant disc space
narrowing compared to preoperative disc height was observed during the time period (p<0.05). The histological
grade, collagen type 1 and 2, aggrecan, and matrix metalloprotease-13 mRNA expression and Hematoxylin and
Eosin were indicative of degeneration, supporting the results of the radiology. Conclusion: Administration of
sulforaphane and riboceine in this study revealed the regenerative potential of nutritional supplement in
intervertebral disc. The annular puncture model can be used to study and validate the therapeutic potential of drugs
and biological materials in the treatments of degenerative disc disease.
Description
Scholarly articles
Keywords
Animal model , Intervertebral disc degeneration , Sulforaphane , Riboceine , Radiology , Histology , Research Subject Categories::MEDICINE
Citation
Ogunlade B, Osinubi AA., Duru FIO, Adelakun SA, Ogunlade SA, & Alao AA. (2017). Histomorphological response of sulforaphane and riboceine on annular puncture-induced model of rabbits intervertebral disc degeneration. European Journal of Biomedical and Pharmaceutical Sciences (ejbps), 4(11):01-09. ISSN 2349-8870.