Novel method for the determination of haemoglobin phenotypes by cyclic voltammetry using glassy carbon electrode

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dc.contributor.authorOgunlesi, M.
dc.contributor.authorOkiei, W.O.
dc.contributor.authorAkanmu, A.S.
dc.contributor.authorPopoola, T.
dc.contributor.authorOkafor, K.
dc.date.accessioned2019-08-23T14:55:19Z
dc.date.available2019-08-23T14:55:19Z
dc.date.issued2009-12-01
dc.description.abstractHaemoglobin is the oxygen transporter within the red blood cell. The oxygenation reaction is accompanied by release or uptake of protons depending on the pH. The electroactive nature of the reaction between haemoglobin and oxygen renders the binding study suitable for investigation by electrochemical method. Haemoglobin samples prepared from blood samples of known phenotypes were diluted with 0.2M acetate buffer, pH 5.0, to give stock solutions of concentration 2 x 10-5 mol.Fe/dm3. Aliquots were drawn and diluted with the buffer and cyclic voltammetry was carried out under nitrogen using glassy carbon/Ag, AgCl/Pt electrode system. 1.4 x 10-7 mol.Fe.dm-3. The values of peak current obtained were in the range of 3.1 – 3.6 µA for HbA, 1.7 – 2.2 µA for HbAS, 1.2 – 1.6 µA for HbS and 2.5 – 3.00 µA for HbAC in the fixed range of concentrations of the haemoglobins. These values were used to identify the phenotypes of several haemoglobins successfully. The values of the peak current are sufficiently distinct to allow for rapid determination of these phenotypes. The method is simple and faster than the conventional electrophoretic method and removes the need to use standards once the characteristic values of peak current have been established. The good correlation between peak current and concentration also provides an accurate determination of the concentrations of haemoglobin samples.en_US
dc.identifier.citationOgunlesi, M., Okiei, W., Akanmu, A. S., Popoola, T., Okafor, K., & Akore, O. (2009). Novel method for the determination of haemoglobin phenotypes by cyclic voltammetry using glassy carbon electrode. Int. J. Electrochem. Sci, 4, 1593-1606.en_US
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/4655
dc.language.isoenen_US
dc.publisherInternational Journal of Electrochemical Scienceen_US
dc.subjectResearch Subject Categories::NATURAL SCIENCES::Chemistry::Analytical chemistry::Electrochemistryen_US
dc.subjectphenotypesen_US
dc.subjectcyclic voltammetryen_US
dc.subjectglassy carbon electrodeen_US
dc.titleNovel method for the determination of haemoglobin phenotypes by cyclic voltammetry using glassy carbon electrodeen_US
dc.typeArticleen_US
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