In Vitro Cytotoxic Effect of andrographolide On MDA-MB-231-LM2 breast cancer cells and its formulation and characterization as an emulsion

Simple item page
dc.contributor.authorOseni, B.A.
dc.contributor.authorAzubuike, C.P.
dc.contributor.authorOkubanjo, O.O.
dc.contributor.authorIgwilo, C.I.
dc.date.accessioned2020-02-24T11:21:27Z
dc.date.available2020-02-24T11:21:27Z
dc.date.issued2019-11
dc.descriptionStaff publicationsen_US
dc.description.abstractAndrographolide, a potential chemotherapeutic agent elicits its anticancer activity on various cancer types by activating multiple cell death pathways thereby possessing the intrinsic ability to combat resistance. The andrographolide molecule however is poorly soluble in water (74 μg/mL) resulting in reduced efficacy; hence, the need for its formulation into appropriate dosage form. The study is aimed at investigating the in vitro cytotoxic potency of andrographolide on MDA-MB-231-LM2 breast cancer cells with subsequent formulation and characterization of andrographolide emulsions. In vitro cytotoxic effect of andrographolide on MDA-MB-231-LM2 breast cancer cells was investigated via the MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay, andrographolide was formulated as an emulsion using olive oil, castor oil and liquid paraffin respectively. The formulations were characterized by determining the emulsion type, particle size, flow rate, viscosity and andrographolide content. Andrographolide was found to be cytotoxic to MDA-MB-231-LM2 breast cancer cells having an IC50 (concentration required to cause 50% reduction in viable cells) value of 25.96 μM. The formulations contained particles between 6.53 to 14.4 μm size; formulations containing olive oil and liquid paraffin had smaller particle size and higher viscosity than the castor oil formulation. The olive oil emulsion had lower flow rate. All formulations were of the oil-in-water emulsion type containing 98.3 – 104.2% andrographolide. Andrographolide exhibits cytotoxic activity on MDA-MB-231-LM2 cells. Its emulsion formulations containing olive oil and liquid paraffin as oil phase possess desirable physicochemical properties than the castor oil formulation.en_US
dc.identifier.citationOseni B. A., Azubuike C. A., Okubanjo O. O., & Igwilo C. I. (2019). In Vitro Cytotoxic Effect of Andrographolide On MDA-MB-231-LM2 Breast Cancer Cells and Its Formulation and Characterization As An Emulsion. Trop J Nat Prod Res. 3(11): 319-325.en_US
dc.identifier.otherdoi.org/10.26538/tjnpr/v3i11.1
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7830
dc.language.isoenen_US
dc.publisherNatural Product Research Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.en_US
dc.relation.ispartofseriesTrop J Nat Prod Res.;Vol.3(11)
dc.subjectAndrographolideen_US
dc.subjectMDA-MB-231-LM2 cellsen_US
dc.subjectCytotoxic effecten_US
dc.subjectBreast canceren_US
dc.subjectEmulsionen_US
dc.subjectResearch Subject Categories::PHARMACYen_US
dc.titleIn Vitro Cytotoxic Effect of andrographolide On MDA-MB-231-LM2 breast cancer cells and its formulation and characterization as an emulsionen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
In Vitro Cytotoxic Effect of Andrographolide Emulsion.pdf
Size:
969.23 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pharmaceutics and Pharmaceutical Technology- Scholarly Publications