Characterisation of a Neutral Protease Produced by Micrococcus Luteus

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dc.contributor.authorIlori, M.O
dc.contributor.authorAmund, O.O
dc.contributor.authorOmidiji, O
dc.date.accessioned2016-06-06T13:31:40Z
dc.date.available2016-06-06T13:31:40Z
dc.date.issued1995-05-26
dc.descriptionStaff Publicationen_US
dc.description.abstractA proteolytic enzyme produced by a cassava-fermenting strain of Micrococcus luteus was extracted and purified 50-fold by gel filtration and ion exchange chromatography. The optimum pH for the enzyme was 7.0, the optimum temperature 25o C, the apparent molecular weight 42 kDa and the Km value, 0.45 mg ml-1 with casein as substrate. The enzyme was stimulated by Ca2+ and Mg2+ but inhibited by Zn2+ and Co2+ ions. Other inhibitors were EDTA, KCN, citric acid and L-cysteine indicating the enzyme to be a metalloprotease.en_US
dc.identifier.citationIlori, M.O, Amund, O.O and Omidiji, O. (1995) Characterisation of a Neutral Protease Produced by Micrococcus Luteus.en_US
dc.identifier.urihttp://www.degruyter.com/dg/viewarticle.fullcontentlink:pdfeventlink/$002fj$002fznc.1996.51.issue-5-6$002fznc-1996-5-623$002fznc-1996-5-623.pdf?t:ac=j$002fznc.1996.51.issue-5-6$002fznc-1996-5-
dc.identifier.urihttp://ir.unilag.edu.ng:8080/xmlui/handle/123456789/733
dc.language.isoenen_US
dc.subjectPurificationen_US
dc.subjectInhibitorsen_US
dc.titleCharacterisation of a Neutral Protease Produced by Micrococcus Luteusen_US
dc.typeArticleen_US
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