Electrochemical Quantification of The Levels of Hydrogen Peroxide in Cassava Using Glassy Carbon Electrode Modified with Chitosan/Silver NanoHybrid
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Date
2020
Authors
Akitoye, A.A
Ibrahim, G.O
Okiei, W.O
Journal Title
Journal ISSN
Volume Title
Publisher
The Proceedings of The Nigerian Academy of Science
Abstract
This study correlates the production of hydrogen peroxide in cassava with its rapid postharvest
physiological deterioration (PPD). Chitosan/silver nanohybrid was synthesized and immobilized
on glassy carbon electrode for improved detection of hydrogen peroxide in electrochemical
studies. The cathodic peak current for the reduction of hydrogen peroxide to water and oxygen
occurred at -550 mV. β-carotene contents of the cassava cultivars were quantified using UV-Vis
spectroscopy at a wavelength of 480 nm. No significant amount of hydrogen peroxide was found
in the root tubers on the first, second, third and fourth day. However, the production of hydrogen
peroxide from the different cultivars on the fifth and sixth day after harvest was found to correlate
with their respective β-carotene contents. The cultivar with the highest β-carotene content (Yellow
roots- IBA070593: 0.0044 mg/g) was found to have the lowest level of hydrogen peroxide on day
5 and day 6: 0.096 mmol/g FW and 0.037 mmol/g respectively; while that with the least β-carotene
level (White roots- IBA980505: 0.0000 mg/g) demonstrated the highest level of hydrogen peroxide
content on day 5 and day 6: 0.177 mmol/g FW and 0.096 mmol/g respectively; and highest
percentage increase from the fifth to the sixth day of the PPD process. It is seen from this study
that an increase in the level of hydrogen peroxide indicates PPD, and that antioxidants with
hydrogen peroxide scavenging properties can help increase shelf-life of cassava cultivars.
Keywords: Cassava, food security, postharvest physiological deterioration
Description
Scholarly article
Keywords
Cassava , Electrochemical , Silver Nanoparticle , Hydrogen Peroxide , Betacarotene , Research Subject Categories::NATURAL SCIENCES
Citation
Akitoye, A. A., Ibrahim, G. O., & Okiei, W. O. (2021). Electrochemical quantification of the levels of hydrogen peroxide in cassava using glassy carbon electrode modified with chitosan/silver nano-hybrid. Proceedings of the Nigerian Academy of Science, 13(2)