Novel laccase from Xylaria polymorpha and its efficiency in the biotransformation of pharmaceuticals: Optimization of operational conditions, comparative effect of redox-mediators and toxicity studies
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Date
2022
Authors
Bankole, P.O.
Omoni, V.T.
Tennison-Omovoh, C.A.
Adebajo, S.O.
Mullae, S.I.
Adekunle, A.A.
Semple, K.T.
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Elsevier
Abstract
The promising potentials of biocatalytic treatment processes in the removal of micropollutants whilst eliminating health and environmental hazards have attracted great attention in recent years. This current work investigated the biotransformation efficiency of a novel laccase from Xylaria polymorpha (XPL) in comparison with com- mercial laccases from Trametes versicolor (TVL) and Aspergillus sp. (ASL). XPL exhibited better oxidation per- formance (95.7%) on AMX than TVL (92.8%) and ASL (90.5%). Optimization of operational conditions revealed that AMX was best oxidized at pH 5, temperature (30 ◦C), and concentration (1.0 mg L 1). The investigation carried out to determine the effect of redox mediators revealed violuric acid (VLA) as the best redox mediator.
The laccase stability experiments elucidated that the oxidation of AMX is time and mediator concentration dependent with ABTS exhibiting highest deactivation of XPL active sites. Two metabolic products; amoxicillin penilloic acid and 5-hydroxy-6-(4-hydroxyphenyl) 3-(1,3-thiazolidin-2-yl)piperazin-2-one of AMX were ob- tained through Liquid Chromatography-Mass Spectrometry (LC-MS) analyses. The toxicity assessments carried out after oxidation of AMX by XPL showed 94% and 97% reduced toxicity on Artemia salina and Aliivibrio fischeri respectively. The study further underscored the efficiency of biocatalytic-mediator technology in the trans- formation of complex micropollutants into less toxic substances in an eco-friendly wa
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Bankole, P.O. , Omoni ,V.T. , Chidinma Angela Tennison-Omovoh, Seun Owolabi Adebajo, Sikandar Imamsab Mulla, Adedotun Adeyinka Adekunle, Kirk Taylor Semple (2022). Novel laccase from Xylaria polymorpha and its efficiency in the biotransformation of pharmaceuticals: Optimization of operational conditions, comparative effect of redox-mediators and toxicity studies.Colloids and Surfaces B: Biointerfaces 217 (2022) 112675