Molecular-based analysis of the bacterial community structure of spoilt latex paints in Nigeria

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dc.contributor.authorObidi, O.F.
dc.contributor.authorWenner, S.
dc.contributor.authorHalverson, L
dc.date.accessioned2019-09-05T08:44:23Z
dc.date.available2019-09-05T08:44:23Z
dc.date.issued2017-02
dc.description.abstractBiodeteriorated paints showing discolouration, loss of viscosity and foul odour have resulted in substantial economic losses in Nigeria. The present study focused on enumeration and identification of bacteria isolated from spoilt paints made in Nigeria using a non-selective nutrient agar (NA) and a semi-selective Pseudomonas Isolation Agar (PIA). The bacterial population density in a fresh paint sample was 3.0 x 10 3 CFU/ml while that of five samples of spoilt paint ranged from 1.0 x 10 3 to 1.2 x 106 CFU/ml on NA. The fresh paint had no growth on PIA while spoilt paint bacterial counts ranged from 2.0 x 10 3 to 8.0 x 10 5 CFU/ml. The most diverse colony types from the spoilt paint samples were randomly selected for purification. Nine isolates were subjected to sequencing of a portion of the 16S rRNA gene and 14 isolates to whole-cell fatty acid methyl ester profiling using the MIDI-FAME system. The most prevalently identified were Klebsiela and Providencia species. A subset of isolates was examined by transmission electron microscopy. Other detected genera included Bacillus, Brevibacillus, Lysinibacillus, and the Nocardioforms. Our results show that spoilt latex paints made in Nigeria can harbour diverse bacterial species which can cause biodeteriorationen_US
dc.identifier.citationObidi, O. F., Wenner, S., & Halverson, L. (2017). Molecular-based analysis of the bacterial community structure of spoilt latex paints in Nigeria. The Egyptian Society of Experimental Biology 13(2), 145 – 149en_US
dc.identifier.issn2090-0503
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/5129
dc.language.isoenen_US
dc.publisherThe Egyptian Society of Experimental Biologyen_US
dc.subjectBiodeteriorated paints, 16S rRNA, molecular methods, PCR, FAMEen_US
dc.titleMolecular-based analysis of the bacterial community structure of spoilt latex paints in Nigeriaen_US
dc.typeArticleen_US
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