Using DNA-based techniques to identify hybrids among linear-leaved Potamogeton plants collected in China
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Date
2010
Authors
Du, Z.
Yang, C.
Chen, J.
Guo, Y.
Kadiri, A. B.
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley-Blackwell
Abstract
It is well known that interspecific hybrids occur in the genus Potamogeton. The linear-leaved Potamogeton
species commonly have highly variable morphological characteristics. Their hybrids often show similar vegetative
characters to their parental species and their identification based solely on morphology is not always conclusive. In
order to clarify whether there are any hybrids from the linear-leaved Potamogeton plants collected in China, we used
internal transcribed spacers (ITS) of nuclear ribosomal DNA and chloroplast rbcL gene sequences to identify the
hybrids. Using ITS sequence additivity, we identified four hybrids, namely P. orientalis (P. pusillus × P. oxyphyllus),
P. pusillus × P. berchtoldii, P. foliosus × P. octandrus, and P. cristatus × P. octandrus. The latter three hybrids
should be considered as new hybrids in Potamogeton. The maternal parents of the four hybrids were confirmed using
chloroplast rbcL gene sequences.
Description
Keywords
hybridization , internal transcribed spacer , Potamogeton , rbcL
Citation
Du, Z., Yang, C., Chen, J., Guo, Y. and Kadiri, A. B. (2010). Using DNA-based techniques to identify hybrids among linear-leaved Potamogeton plants collected in China. Journal of Systematics and Evolution 48 (4): 265–270