Purification and Properties of a Neutral Protease Produced by Lactobaccillus Brevis
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Date
1990
Authors
Amund, O.O
Omidiji, O
Ilori, M.O
Journal Title
Journal ISSN
Volume Title
Publisher
Elservier
Abstract
An extracellular glucose-forming amylase was produced by Lactobacillus brevis isolated from ‘Kagasok tea’. The enzyme was purified 70-fold and had optimal activity at 55 oC and pH 6.5. Its Km value for starch was 0.27 mg ml-1 and its M, was approx. 75,900 Da. The activity of the enzyme was enhanced by Ca2+, Mg2+, Na+ or K+ and inhibited by EDTA, KCN, citric acid and L-cysteine.
Description
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Keywords
Tempreture , Enzyme
Citation
Amund, O.O, Omidiji, O and Ilori, O (1990) Purification and Properties of a Neutral Protease Produced by Lactobaccillus Brevis. Journal of Biotechnology (13)