Encapsulation of Andrographolide in Poly(lactide-co-glycolide) Nanoparticles: Formulation Optimization and In Vitro Efficacy Studies
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Date
2021
Authors
Oseni, B.A
Azubuike, C.P
Okubanjo, O.O
Igwilo, C.I
Panyam, J
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers in Bioengineering and Biotechnology
Abstract
Andrographolide is a potential chemopreventive and chemotherapeutic agent that
suffers from poor aqueous solubility. Encapsulation in poly(lactide-co-glycolide) (PLGA)
nanoparticles can overcome solubility issues and enable sustained release of the
drug, resulting in improved therapeutic efficacy. In this study, andrographolide was
encapsulated in PLGA nanoparticles via emulsion solvent evaporation technique. Effect
of various formulation parameters including polymer composition, polymer molecular
weight, polymer to drug ratio, surfactant concentration and the organic solvent used
on nanoparticle properties were investigated. A selected formulation was used to
determine the effect of encapsulation in nanoparticles on andrographolide’s in vitro
anticancer efficacy. Nanoparticles formulated using a polymer with 85:15 lactide to
glycolide ratio and ethyl acetate as the organic solvent were found to be optimal based
on average hydrodynamic particle size (135 ± 4 nm) and drug loading (2.6 ± 0.6%w/w).
This formulation demonstrated sustained release of andrographolide over 48 h and
demonstrated significantly greater in vitro anticancer efficacy compared to free drug in a
metastatic breast cancer cell line. These results suggest that additional, more in-depth
efficacy studies are warranted for the nanoparticle formulation of andrographolide.
Description
Scholarly articles
Keywords
andrographolide , poly(lactide-co-glycolide) , nanoparticles , formulation optimization , breast cancer , Research Subject Categories::MEDICINE
Citation
Oseni B.A., Azubuike C.P., Okubanjo O.O., Igwilo C.I., Panyam J. (2021). Encapsulation of Andrographolide in Poly(lactide-co-glycolide) Nanoparticles: Formulation Optimization and In Vitro Efficacy Studies. Front. Bioeng. Biotechnol, 9:639409. doi:10.3389/fbioe.2021.639409