Pectinase Production by Bacillus Megaterium, Bacillus Bataviensis, and Paenibacillus sp. Isolated from Decomposing Wood Residues in the Lagos Lagoon

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dc.contributor.authorAkinyemi, B.T
dc.contributor.authorBuraimoh, O.M
dc.contributor.authorOgunrinde, O.O
dc.contributor.authorAmund, O.O
dc.date.accessioned2017-10-13T08:15:52Z
dc.date.available2017-10-13T08:15:52Z
dc.date.issued2017-09
dc.descriptionStaff Publicationsen_US
dc.description.abstractThree wood decomposing bacteria isolated from the Lagos lagoon, Bacillus megaterium, Bacillus bataviensis and Paenibacillus sp. were screened for their pectinase producing abilities using pectin as substrate under submerged fermentation (SMF) conditions. The results showed that all three isolates produced appreciable pectinolytic activities. Paenibacillus sp. showed the highest pectinase activity when compared with the other two isolates. The optimum pH for pectinase activity for both B. megaterium and B. bataviensis was 8.0 while it was 6.5 for Paenibacillus sp., B. bataviensis, and B. megaterium showed optimum pectinase activity at 60°C and Paenibacillus sp. at 40°C. Metal ions such as Na+ and K+ improved the activity of pectinase produced by the three isolates when compared to the effect of Zn2+ and Mn2+. The molecular weights of the enzymes were also estimated by gel filtration as 29,512 da, 32,359 da, and 25,119 da for Paenibacillus sp., B. megaterium and B. bataviensis respectively. The study has provided a platform for further investigation into the biochemical characterization of the enzyme, and optimization of culture conditions to scale up pectinase production for commercial exploitation.en_US
dc.identifier.citationAkinyemi, B.T Et...al (2017) Pectinase Production by Bacillus Megaterium, Bacillus Bataviensis, and Paenibacillus sp. Isolated from Decomposing Wood Residues in the Lagos Lagoon. The Journal of Tropical Science, Vol. 7 (3), 204-207pp.en_US
dc.identifier.uriwww.jtrolis.ub.ac.id/index.php/jtrolis/article/download/723/pdf
dc.identifier.urijtrolis.ub.ac.id
dc.identifier.urihttp://ir.unilag.edu.ng:8080/xmlui/handle/123456789/2193
dc.language.isoenen_US
dc.subjectBacteriaen_US
dc.subjectWood Residueen_US
dc.subjectLagos Lagoonen_US
dc.titlePectinase Production by Bacillus Megaterium, Bacillus Bataviensis, and Paenibacillus sp. Isolated from Decomposing Wood Residues in the Lagos Lagoonen_US
dc.typeArticleen_US
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