High proportion of carbapenemase producing Escherichia coli and Klebsiella pneumoniae among extended spectrum β-lactamase producers in Nigerian

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dc.contributor.authorOlalekan, A
dc.contributor.authorOnwugamba, F
dc.contributor.authorIwalokun, B
dc.contributor.authorMellmann, A
dc.contributor.authorBecker, K
dc.contributor.authorSchaumburg, F
dc.date.accessioned2021-07-03T02:45:47Z
dc.date.available2021-07-03T02:45:47Z
dc.date.issued2019-09
dc.description.abstractObjectives: Carbapenem-resistant Enterobacterales are a global problem, however little is known about the burden and origin of carbapenem resistance in Africa. The objectives of this study were to determine the proportion of carbapenem-resistant isolates among extended-spectrum β-lactamase-producing Enterobacterales (ESBL-E), to identify the underlying mechanisms of resistance and to assess the population structure of carbapenem-resistant isolates from Nigeria. Methods: ESBL-E isolates (n = 175) from infections were collected at four hospitals in Lagos, Nigeria, from July 2016 to January 2018 and were screened for carbapenem resistance using a VITEK12 automated system. All carbapenem-resistant ESBL-E (CRE) were screened for blaKPC, blaCTX-M, blaCMY-2, blaNDM, blaVIM, blaIMP, blaOXA-181 and blaOXA-48 genes. Genotyping of randomly selected isolates was performed by whole-genome sequencing. Results: The isolates included Escherichia coli (n = 113; 64.6%) and Klebsiella pneumoniae (n = 62; 35.4%). Of the 175 ESBL-E isolates, 48 (27.4%) were resistant to carbapenems (15 E. coli and 33 K. pneumoniae). CRE isolates carried blaNDM (n = 30; 62.5%), blaNDM + blaOXA-181 (n = 10; 20.8%), blaOXA-181 (n = 2; 4.2%) and blaNDM + blaOXA-48 (n = 1; 2.1%); no carbapenemase gene was detected in 5 isolates (10.4%). The isolates showed low diversity and were mainly associated with multilocus sequence typing (MLST) sequence types ST410 for E. coli and ST395 and ST147 for K. pneumoniae. Conclusion: Carbapenem resistance is frequent among ESBL-E in Nigeria and is mainly associated with blaNDM. Genotyping suggested that the observed clones possibly originated from Southeast Asia.en_US
dc.description.sponsorshipThis study was supported by the Cooperation Visits Pro- gramme of the Deutsche Forschungsgemeinschaft and The World Academy of Sciences (DFG-TWAS) [SCHA 1994/4-1]. The funder was not involved in the conduct of the study or publication of the results.en_US
dc.identifier.otherhttps://doi.org/10.1016/j.jgar.2019.09.007;
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/9465
dc.language.isoenen_US
dc.publisherJournal of Global antimicrobial Resistanceen_US
dc.relation.ispartofserieshttps://doi.org/10.1016/j.jgar.2019.09.007;
dc.subjectcarbapenemase-producing Escherichia coli and Klebsiella pneumoniaeen_US
dc.titleHigh proportion of carbapenemase producing Escherichia coli and Klebsiella pneumoniae among extended spectrum β-lactamase producers in Nigerianen_US
dc.typeArticleen_US
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