A comparison of antioxidant and Fourier Transform Infra Red Spectroscopy (FTIR) analysis on extracts of syzygium guineeses (Myrtaceae)

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Shonekan OO, Otuka AC, Adeyemi D.K, and Fatunsi OT
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Journal of Basic and social Pharmacy Research
Background: Syzygium guineenses, (the most common and abundant specie in Nigeria) is a medicinal plant used by traditional practitioners in northern Nigeria for a variety of healing purposes. Objective: The main objective of this project was to carry out a comparison of antioxidant activities and Fourier transform Infrared Spectrophotometric (FTIR) analysis on both methanol and hexane leaf extracts of S. guineenses. Methods: Phytochemical screening, semi-quantitative, DPPH (1,1-diphenyl-2-picrylhydrazyl)- dot blot assay and FTIR analysis were performed on both extracts to determine antioxidant activity and identify the functional groups present. Results: Phytochemical screening revealed the presence of alkaloids, reducing sugars and cardiac glycosides, which were observed to be more prominent in the methanol extract than hexane. The in vitro antioxidant assay also revealed a more intense yellow colour of inhibition in methanol extract than the hexane extract. The FTIR spectra revealed different characteristic peak values with various functional compound in both extracts. The methanol extract displayed major peaks of absorption at 3341cm-1 (-OH) for alcohol, 1736 cm-1 (C=O) carbonyl group, 1161.83 cm-1, 1036.49 cm-1 (C-O) of esters. Other absorption bands like 1452.25 cm-1 and 1612.20 cm-1for alkenes were present in both extracts. Conclusion: This result shows that the methanol extract of S. guineenses has a higher potential of phytochemicals, antioxidants and functional groups than the hexane extract.
The authors declare no conflict of interest and no source of grant for the completion of this project. The authors would like to thank the staff of the Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Department of Chemistry, Faculty of Science, University of Lagos, Nigeria.
FTIR, S. guineenses, DPPH-Dot-blot assay, phytochemicals