Liquid Chromatographic Technique for the Simultaneous Determination of Sulphamethoxazole and Trimethoprim in Pharmaceutical Formulations

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dc.contributor.authorOlusegun, A.O
dc.contributor.authorHamed, N.C
dc.contributor.authorOlajire, M
dc.contributor.authorOladipo, O.J
dc.date.accessioned2019-03-04T13:47:44Z
dc.date.available2019-03-04T13:47:44Z
dc.date.issued2016
dc.descriptionJournal Articlesen_US
dc.description.abstractThis paper describes the development and validation of a simple, specific, precise, and accurate Liquid Chromatographic method for the simultaneous determination of sulphamethoxazole and trimethoprim in pharmaceutical dosage forms. The chromatographic resolution was achieved with 50mM sodium phosphate buffer and acetonitrile (85:15) on a reversed phase column - Octyldecylsilane C18 column (100 x 4.6 mm, i.d., 5μm) - at ambient temperature. The flow rate through the column was 1ml/min and the UV detection was at 260nm using Agilent HPLC 1100LC System. The mean retention times for trimethoprim and sulphamethoxazole were 2.998 and 6.205 minutes respectively. Calibration curves were rectilinear over the ranges 5–80 mg/L (trimethoprim) and 25–400 mg/L (sulphamethoxazole). The RSD was less than 2.61% and percentage recovery was between91.93% - 103.98% with respect to precision and accuracy. The method has been used to analyze brands of cotrimoxazole tablets. The percentage content of sulphamethoxazole and trimethoprim were found to be comparable with BP 2002 requirement. This method, which has a fair run time of 6 minutes, is cost effective for routine analytical work and for quality control and product monitoring. Keywords:en_US
dc.description.sponsorshipTertiary Education Trust Funden_US
dc.identifier.citationOlusegun, A.O, Hamed, N.C, Olajire, M and Oladipo, O.J (2016). Liquid Chromatographic Technique for the Simultaneous Determination of Sulphamethoxazole and Trimethoprim in Pharmaceutical Formulations. Unilag Journal of Medicine, Science and Technology, Vol.4(2), 120-132p.en_US
dc.identifier.issn2408-5049
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/3881
dc.language.isoenen_US
dc.publisherUniversity of Lagos Press, Akokaen_US
dc.relation.ispartofseriesUniversity of lagos Journal;Vol.4(2)
dc.subjectSulphamethoxazoleen_US
dc.subjectTrimethoprimen_US
dc.subjectHPLCen_US
dc.subjectPharmaceutical formulationsen_US
dc.titleLiquid Chromatographic Technique for the Simultaneous Determination of Sulphamethoxazole and Trimethoprim in Pharmaceutical Formulationsen_US
dc.typeArticleen_US
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Volume 4, Issue 2, 2016