Effect of some physico-chemical parameters on the production of hydrolytic cell wall degrading enzymes by 2 strains Fusarium oxysporum f. sp. elaeidis
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Ekhorutomwen, O. E.
International Journal of Biotechnology and Food Science
Extracellular endoglucanase, exoglucanase, β-glucosidase, and endo-polymethylgalacturonase enzyme activities were detected in culture filtrates of two pathogenic strains of Fusarium oxysporum f. sp. elaeidis of oil palm. The effect of carbon source, incubation period, pH and temperature were used to detect the production of these enzymes. Although there were no significant differences in the means of enzymes between the two carbon sources used, pectin medium enhanced higher production of endoglucanase and exoglucanase, while β-glucosidase and pectinase show higher production in carboxymethylcellulose medium. On the effect of incubation period on enzymes production, the optimum production of these enzymes was achieved on both 4th and 8th day. Optimum production of enzymes occurred at pH 5.5 except for β-glucosidase which optimum production was pH 7.5. Lastly, optimum temperature for the production of these enzymes was 30°C. Result from this study shows that physico-chemical parameters such as carbon sources, incubation period, pH and temperature influenced the production of these enzymes.
Endoglucanase, exoglucanase, β-glucosidase, endo-polymethylgalacturonase, Fusarium oxysporum f. sp. elaeidis.