Phylogenetic and Genomic relationships among melon populations based on RAPD

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Oshingboye, A. D.
Adeyemi, T. O.
Ogundipe, O.T.
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Asian Network for Scientific Information
Melons belong to the family Cucurbitaceae; it is one of the most important cash crops globally. Their demand is in a very high quantity, especially here in Nigeria. In order to improve the yield of this highly demanded crop, a study was under taken to ascertain the pattern of its genetic diversity. During this study, eighteen melon cultivars samples grown in south western Nigeria was used viz; Cucumis melo, Citrullus lanatus and Lagenaria siceraria species. They were investigated using both seed morphology and molecular characters. For the seed morphology, the characters revealed that they are sharply pointed with elliptical/oval shape. And analysis of total genomic DNA extracted using CTAB protocol on 1% agarose revealed high molecular weight DNA bands in 88.89% of the samples. While studies on their genetic variation investigated using RAPD analysis, with three primers revealed samples have a wide genetic base with monomorphic bands, which were used to generate UPGMA dendograms. The dendograms showed the examined melons are monophyletic with sample AD001 and AD002 showing distinct morphometric evaluation with 18.00 and 16.94 similarity coefficient respectively and samples AD003 and AD004 have a closer similarity coefficient at 2.06. Basically, in this study, we have investigated the extent of variation and of relatedness among the 18 melon cultivars, which can now afford breeders the opportunity to make selections on how to improve on the breeding of melon cultivars. However, this research serves as a basis for further characterization of the melon population.
Research Subject Categories::NATURAL SCIENCES::Biology::Organism biology::Systematics and phylogenetics , Melon, RAPD, UPGMA, monomorphic
Oshingboye, A. D., Adeyemi, T. O. and Ogundipe, O. T. (2013). Phylogenetic and Genomic Relationship among melon based on RAPD. International Journal of Botany.9: 91 – 95