Antioxidant, antimicrobial and hypoglycaemic activities of the ethanol root extract of Olax subscorpioidea Oliv (Olacaceae)
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Date
2018
Authors
Ayoola G.A.
Adeoye w.
Olowu T.
Adepoju-Bello A.A.
Usman A.
Johnson O.
Journal Title
Journal ISSN
Volume Title
Publisher
West African Journal of Pharmacy
Abstract
Background: Olax subscorpioidea(OS) is a shrub or tree that is widely distributed Africa. It is used in traditional
medicine for the management inflammatory disorders, infectious diseases,diabetes mellitus and mental
illness.
Objectives: The aim of this study was to evaluate the antioxidant, hypoglycemic and antimicrobial properties of
the ethanol root extract of Olax subscorpioidea(EOS).
Methods: Antioxidant activity was determined spectrophotometrically using
DPPH) free radical inhibition and ferric reducing power assay. Total phenolic and flavonoid contents were also
determined. The hypoglycemic potential was assessed by investigating the in vitro inhibitory effect of the
extract on á-amylase. The plant was also screened against some gram negative bacteria (Escherichia coli,
Salmonella paratyphi and Pseudomonas aeruginosa), two Gram positive (Staphylococcus aureus and Bacillus
subtilis) and a fungus, Candida albican susing agar well diffusion method.
Results: EOS exhibited moderate radical scavenging activity against DPPH radical with an IC of 3.29 mg/mL 50
compared to an IC value of 0.017 and 0.019 mg/mL for Vitamin C and E respectively. The ferric reducing power 50
was 114.74 M and 253.6 M Fe(II)/mg for EOS and Vitamin C respectively. The á-amylase inhibitory result
showed that EOS was twice as potent as acarbose. IC values of 186.6 and 391.7μg/mL were recorded for EOS 50
and acarbose respectively. Antimicrobial screening showed that EOS was active against gram positive and not
gram negative bacteria with a zone of inhibition range of 14 -18 mm and also inhibited Candida albicanat
higher concentrations (75 & 150 ìg/ml) with a zone of inhibition range of 14 -16 mm.
Conclusion: The results showed that Olax subscorpioidea possesses moderate free radical scavenging activity
and potent hypoglycemic and antimicrobial (narrow spectrum)
Description
Scholarly articles
Keywords
Antioxidant , Infectious diseases , Acarbose , Medicine , Research Subject Categories::PHARMACY::Pharmaceutical chemistry
Citation
Gloria Ayoola, Waheed Adeoye, Temitope Olowu, Aderonke Adepoju-Bello, AbdulRahman Usman, and Oluwatosin Johnson (2018). Antioxidant, antimicrobial and hypoglycaemic activities of the ethanol root extract of Olax subscorpioidea Oliv (Olacaceae).West African Journal of Pharmacy (2018) 29 (1) 121-129