Metabolism of Chlorinated Biphenyls: Use of 3,3/-and 3,5-Dichlorobiphenyl as Sole Sources of Carbon by Natural Species of Ralstonia and Pseudomona

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dc.contributor.authorAdebusoye, S.A
dc.contributor.authorIlori, M.O
dc.contributor.authorPicardal, F. W
dc.contributor.authorAmund, O.O
dc.date.accessioned2015-12-22T14:52:45Z
dc.date.available2015-12-22T14:52:45Z
dc.date.issued2008
dc.description.abstractRalstonia sp. SA-3, Ralstonia sp. SA-4 and Pseudomonas sp. SA-6 are natural strains with a novel capacity to utilize meta-substituted dichlorobiphenyls (diCBs) hitherto not known to serve as a sole source of carbon and energy for polychlorobiphenyl-degraders. In growth experiments, axenic cultures of isolates grew logarithmically on 3,3’-diCB with generation times that ranged insignificantly (t-test, P>0.05) from 30.4 to 33.8 h. Both 3-chlorobenzoate (3-CBA) and chloride produced as metabolites were recovered in non-stoichiometric quantities. The release of chloride by the cultures lagged substantially, indicating that the initial dioxygenase attack preceded cleavage of carbon-chloride bonds and that chloride must have been released from the chlorinated hydroxypentadienoate. In the case of 3,5-diCB, SA-3 and SA-6 metabolised this substrate primarily to 3,5-CBA. The lack of chloride in the culture media coupled with stoichiometric recovery of 3,5-CBA suggests that growth by these strains occurred predominantly at the expense of the unsubstituted phenyl ring. The unique metabolic properties of these three aerobic isolates point to their potential usefulness as seeds for bioremediation of PCBs polluted environments without the need for repeated inoculation or supplementation by a primary growth substrate such as biphenyl.en_US
dc.identifier.citationAdebusoye, et al. (2008) Metabolism of Chlorinated Biphenyls: Use of 3,3/-and 3,5-Dichlorobiphenyl as Sole Sources of Carbon by Natural Species of Ralstonia and Pseudomonas. Chemosphere 70(4) 656-663.en_US
dc.identifier.urihttps://www.researchgate.net/publication/6132676_Metabolism_of_chlorinated_biphenyls_Use_of_33_'-_and_35-dichlorobiphenyl_as_sole_sources_of_carbon_by_natural_species_of_Ralstonia_and_Pseudomonas
dc.identifier.urihttp://ir.unilag.edu.ng:8080/xmlui/handle/123456789/381
dc.language.isoenen_US
dc.subjectRalstonia sp.en_US
dc.subjectPseudomonas sp.en_US
dc.subjectAxenic culturesen_US
dc.subjectHhydroxypentadienoateen_US
dc.subjectBioremediationen_US
dc.subjectBiphenylen_US
dc.titleMetabolism of Chlorinated Biphenyls: Use of 3,3/-and 3,5-Dichlorobiphenyl as Sole Sources of Carbon by Natural Species of Ralstonia and Pseudomonaen_US
dc.typeArticleen_US
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