New Chromogenic Arginine Anilide Substrates for Trypsin and Thrombin

Okiei, W.O.; Somorin, O.

New Chromogenic Arginine Anilide Substrates for Trypsin and Thrombin

dc.contributor.author	Okiei, W.O.
dc.contributor.author	Somorin, O.
dc.date.accessioned	2019-09-27T07:35:00Z
dc.date.available	2019-09-27T07:35:00Z
dc.date.issued	2003
dc.description.abstract	Fifteen chromogenic anilide substrates, namely N-benzyloxycarbonyl-L-arginine-X-substituted anilides (X= H, F, Cl, Br, I, NO2, CF3, CH3 or OCH3) at the meta or para position of the aniline ring, were synthesized using the procedure earlier reported. These substrates are suitable for studying the electronic effects of substituents on trypsin as well as on thrombin hydrolytic activities. The hydrolysis of these substrates by trypsin or thrombin at 37oC and pH 8.4 was found to proceed at significantly different rates in the following order: L-ZAPA.HCl>L-ZAPT.HCl>L-ZAPIA.HCl>ZAPBA.HCl> L-ZAMT. HCL> L-ZAPNA.HCl>L-ZAPCA.HCl>L-ZAMA.HCl>ZAMNA.HCl> L-ZAMTHA. HCL>L ZAPFA.HCl>L-ZAMCA.HCl>L-ZAA.HCl>L-ZAMFA.HCl>L-ZAMTFA.HCl The Km values obtained for the thrombin-catalyzed hydrolysis of the arginine substrates were generally higher than those of the trypsin-catalyzed hydrolysis of the same anilides.	en_US
dc.identifier.citation	Okiei, W., & Somorin, O. (2003). New Chromogenic Arginine Anilide Substrates for Trypsin and Thrombin. African Journal of Science, 35, 165-172.	en_US
dc.identifier.uri	https://ir.unilag.edu.ng/handle/123456789/6196
dc.language.iso	en	en_US
dc.subject	Research Subject Categories::NATURAL SCIENCES::Chemistry	en_US
dc.subject	anilide	en_US
dc.subject	arginine	en_US
dc.subject	chromogenic	en_US
dc.subject	trypsin	en_US
dc.subject	thrombin	en_US
dc.title	New Chromogenic Arginine Anilide Substrates for Trypsin and Thrombin	en_US
dc.type	Article	en_US