The effect of inoculum size and sublethal injury on the ability of Listeria monocytogenes to initiate growth under suboptimal conditions

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dc.contributor.authorPascual, C.
dc.contributor.authorRobinson, T. P.
dc.contributor.authorOcio, M. J.
dc.contributor.authorAboaba, O.O.
dc.contributor.authorMackey, B. M.
dc.date.accessioned2022-09-06T12:26:51Z
dc.date.available2022-09-06T12:26:51Z
dc.date.issued2001-08-22
dc.descriptionScholarly articleen_US
dc.description.abstractAims: To investigate the effect of inoculum size and physiological state on the ability of Listeria monocytogenes cells to initiate growth under suboptimal conditions of salt concentration and pH. Methods and Results: Cell suspensions were serially diluted in media of different salt concentration or pH and replicate inocula distributed into 96-well microplates. The proportion of wells showing growth at each dilution level was determined after incubation for 6 weeks for each set of conditions. Growth occurred from single cells up to a concentration of 1á2 mol l)1 NaCl; above this threshold, the inoculum size needed to initiate growth became progressively larger. A similar effect was seen with decreasing pH but only very close to the growth/no growth boundary. The threshold for inoculum-dependent growth was lower in exponential phase cells than in stationary phase ones and sublethal injury greatly decreased the probability of growth from small inocula. Conclusions: The growth/no growth boundary for L. monocytogenes is not an absolute cut-off point but represents a region where the probability of growth rapidly decreases as conditions become more extreme. We interpret the requirement for a critical inoculum size for growth as being due to death of a proportion of cells in the inoculum rather than to co-operative population effects. Signi®cance and Impact of the Study: Physiological heterogeneity within the cell population and inoculum size will affect the risk of L. monocytogenes growing in food.en_US
dc.identifier.citationPascual, C., Robinson, T. P., Ocio, M. J., Aboaba, O. O., & Mackey, B. M. (2001). The effect of inoculum size and sublethal injury on the ability of Listeria monocytogenes to initiate growth under suboptimal conditions. Letters in Applied Microbiology, 33(5), 357-361.en_US
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/11316
dc.language.isoenen_US
dc.publisherLetters in Applied Microbiologyen_US
dc.subjectListeria monocytogenes cellsen_US
dc.subjectSalt concentrationen_US
dc.subjectCell suspensionen_US
dc.subjectResearch Subject Categories::NATURAL SCIENCESen_US
dc.titleThe effect of inoculum size and sublethal injury on the ability of Listeria monocytogenes to initiate growth under suboptimal conditionsen_US
dc.typeArticleen_US
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