Cell Biology & Genetics-Scholarly Publications

Permanent URI for this collection

https://ir.unilag.edu.ng/handle/123456789/3375

Browse

Browsing Cell Biology & Genetics-Scholarly Publications by Author "Adebesin, O. A."

Now showing 1 - 1 of 1
  • Item
    Open Access
    ASSESSMENT OF GENETIC DIVERSITY AMONG SELECTED MAIZE LANDRACES AND HYBRIDS USING SSR MARKERS LINKED TO QTLS FOR DROUGHT AND NITROGEN TOLERANCE
    (Nigerian Journal of Genetics, 2018) Oyenike, O. A.; Bhadmus, O. A.; Adebesin, O. A.; Okinedo, U. E.
    In this study, seventeen quantitative trait loci (QTLs) linked simple sequence repeat (SSR) markers of maize were used to assess genetic diversity in selected 20 maize genotypes adapted to Southern Nigeria. The morpho-physiological traits considered include stay-green characteristics, days to anthesis, days to silking and anthesis-silking interval, leaf number and high nitrogen regime, ear setting and yield components, while the maize genotypes comprised of 9 landraces, 5 commercial hybrids grown by local farmers and 6 recently developed IITA hybrids. A total of 57 polymorphic alleles were amplified and the mean allele number was 3.35 and a range of 2 to 5 alleles per locus. The average polymorphic information content (PIC) value of 0.46, with a minimum and maximum of 0.16 and 0.72 were observed. The genetic similarity ranged from 0.45 to 0.91 with a mean of 0.58. The UPGMA dendrogram indicated that the 20 maize genotypes could be divided into two major groups, of which each had two subgroups. Principal component analysis also depicted diversity among the lines. Based on the grouping, lines from landraces were clustered together, while the commercial had close genetic relationship with IITA hybrids. Genetic diversity was moderately high among the landraces than in the hybrids. This study reveals the efficiency of trait-linked SSR markers to estimate the extent of the genetic variation of the maize varieties for drought/nitrogen regime adaptive traits and will be a contribution to further application of marker assisted selection in maize breeding.