Cell Biology & Genetics-Scholarly Publications

Permanent URI for this collection

https://ir.unilag.edu.ng/handle/123456789/3375

Browse

Browsing Cell Biology & Genetics-Scholarly Publications by Author "Adefenwa, M. A."

Now showing 1 - 2 of 2
  • Item
    Open Access
    Identification of single nucleotide polymorphisms in the agouti signaling protein (ASIP) gene in some goat breeds in tropical and temperate climates
    (Springer Sciences _ Molecular Biology Reports, 2013-05) Adefenwa, M. A.; Peters, S.O.; Agaviezor, B.O.; Wheto, M.; Adekoya, K.O.; Okpeku, M.; Oboh, B.O.; Williams, G.O.; Adebambo, O.A.; Singh, M.; Thomas, B.; Donato, M.; Ikhide, G. I.
    The agouti-signaling protein (ASIP) plays a major role in mammalian pigmentation as an antagonist to melanocortin-1 receptor gene to stimulate pheomelanin synthesis, a major pigment conferring mammalian coat color. We sequenced a 352 bp fragment of ASIP gene spanning part of exon 2 and part of intron 2 in 215 animals representing six goat breeds from Nigeria and the United States: West African Dwarf, predominantly black; Red Sokoto, mostly red; and Sahel, mostly white from Nigeria; black and white Alpine, brown and white Spanish and white Saanen from the US. Twenty haplotypes from nine mutations representing three intronic, one silent and five missense (p.S19R, p.N35K, p.L36V, p.M42L and p.L45W) mutations were identified in Nigerian goats. Approximately 89 % of Nigerian goats carry haplotype 1 (TGCCATCCG) which seems to be the wild type configuration of mutations in this region of the gene. Although we found no association between these polymorphisms in the ASIP gene and coat color in Nigerian goats, in-silico functional analysis predicts putative deleterious functional impact of the p.L45W mutation on the basic amino-terminal domain of ASIP. In the American goats, two intronic mutations, g.293G[Aand g.327C[A, were identified in the Alpine breed, although the g.293G[A mutation is common to American and Nigerian goat populations. All Sannen and Sahel goats in this study belong to haplotypes 1 of both populations which seem to be the wild-type composite ASIP haplotype. Overall, there was no clear association of this portion of the ASIP gene interrogated in this study with coat color variation. Therefore, additional genomic analyses of promoter sequence, the entire coding and non-coding regions of the ASIP gene will be required to obtain a definite conclusion.
  • Item
    Open Access
    Inversion Polymorphism in Two Populations of Drosophila melanogaster in Lagos State, Nigeria
    (Faculty of Science, University of Lagos., 2010) Adefenwa, M. A.; Williams, G. O.; Oboh, B.O.; Adekoya, K. O.
    Two different natural populations of Drosophila melanogaster were surveyed to determine the types of inversions present by examining the salivary gland chromosomes of third instar larvae. Seven different inversions were detected in populations, Tejuosho market and Bariga market (Lagos, south-western Nigeria) populations. All inversions detected were single, paracentric and autosomal. The inversions observed were distributed in the arms of the chromosomes as follows: two in the left arm of chromosome 2 (2L), one in the right arm of chromosome 2 (2R), two in the left arm of chromosome 3 (3L) and two in the right arm of chromosome 3 (3R). Some inversions occurred together with other inversions in the same larva and this occurrence was probably just due to chance but also indicates that such conditions were not lethal. Some of the inversions detected in this study had been recorded by earlier researchers in some parts of south-eastern Nigeria and south-western Nigeria. The occurrence of similar inversions in these regions may be evidence that the inversions are not recent. This similarity in inversions also suggests that there is or had been migration from one place to the other or the inversions originated somewhere else and had been transported to other places.