Biochemistry-Scholarly Publications

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Browsing Biochemistry-Scholarly Publications by Author "Adenekan, S.O."

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    Open Access
    Antisickling and toxicological profiles of leaf and stem of Parquetina nigrescens L.
    (Academic Journals, 2010-04-18) Imaga, N.O.A.; Gbenle, G.O.; Okochi, V.I.; Adenekan, S.O.; Edeoghon, S.O.; Kehinde, M.O.; Bamiro, S.B.; Ajiboye, A.; Obinna, A.
    Folk medicine reportedly uses Parquetina nigrescens L. (Asclepiadaceae) as a herbal remedy for the management of sickle cell anemia. This study was carried out to screen the leaves and stem of P. nigrescens for antisickling activity, erythrocyte membrane -stabilizing effects and any end organ toxicity. Percentage reversal and inhibition of sickling parameters were analyzed on pre-sickled HbSS blood cell suspensions using sodium metabisulphite solution as inducer and 5 mg/ml parahydroxybenzoic acid and normal saline as positive and negative controls respectively. Effects of the plant extracts on the erythrocyte were assessed using osmotic fragility and the toxicity profile done via LD50 and sub-acute toxicity studies on graded concentrations of extract. Results show that P. nigrescens has appreciable antisickling activity, has no toxic effect when administered at low concentrations and protects the integrity of the erythrocyte membrane as evidenced in the fragiliogram by the reduction in hemolysis of the Hbss cells.
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    Open Access
    Assessment of antioxidation potential of selected plants with antisickling property
    (Academic Journals, 2010-11-04) Imaga, Ngozi O. A.; Adenekan, S.O.; Yussuph, G.A.; Nwoyimi, T.I.; Balogun, O.O.; Eguntola, T.A.
    Methanol extracts of herbs hitherto reported to have antisickling activity namely, Carica papaya leaf extract, Fagara zanthoxyloides root extract, Cajanus cajan seed extract and Parquetina nigrescens leaf extract, were evaluated in this study. An assessment of their antioxidation potential was determined by assaying for their phytochemical constituents, total phenol content, scavenging activity on 2,2- diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant status through the ferric thiocyanate method. The extracts have similar phytochemical constituents and exhibited high scavenging activity compared to gallic acid and ascorbic acid standards due to their relatively high total phenol content. These findings suggest that C. papaya leaf extract, F. zanthoxyloides root extract, C. cajan seed extract and P. nigrescens leaf extract are endowed with antioxidant phytochemicals which may act singly or synergistically to potentiate the antisickling action of the plants.
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    Open Access
    SAPONIN, PREPONDERANT PHYTOCHEMICAL IN CARICA PAPAYA LEAF EXTRACT, POSSIBLY RESPONSIBLE FOR ITS REPORTED ANTISICKLING & ANTIOXIDANT ACTIVITY
    (UNILAG Journal of Medicine, Science and Technology, 2013) Imaga, N.O.A.; Adenekan, S.O.; Fakanye, T.J.; Olaniyan, F.K.; Alakaloko, A.A.
    This study examined the preponderant phytochemicals and antioxidant potential in the extract fractions of Carica papaya leaf to ascertain possible mechanisms of action of the plant’s earlier reported antioxidant and antisickling properties. Soxhlet extraction of the C. papaya ground leaves gave rise to the initial crude methanolic extract of the papaya leaf and subsequent exhaustive fractionation using a separatory funnel with chloroform, ethylacetate and butanol organic solvents were done to obtain the desired extract fractions. Determination of quantitative and qualitative phytochemical constituents of C.papaya leaf extract and determination of antioxidant property of the different fractions were done using standard protocols. The results showed that Saponin was the most abundant phytochemical in the chloroform, butanol and water-soluble fractions but not the ethyl acetate fraction. While the antioxidant assay showed that the chloroform fraction had the highest antioxidant property. The result of this study supports earlier findings on the rich antioxidant property of the C. papaya leaf crude extract and specifically pinpoints the type of phytochemical and fraction that may possess more of this antioxidant property.