Biochemistry-Scholarly Publications

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    Open Access
    INTERPERITONEAL VS ORAL ROUTE OF ALCOHOL ADMINISTRATION,WHICH IS BETTER?
    (Global Research Journal of Microbiology, 2013) Ebuehi, O.A.T.; Dawodu, O.G.; Odesanmi, O.S.; Buari, O.S.
    The major problem researchers of alcohol intoxication and its dependence face is the route of administration as animals though good do not mimic the perfect subjects i.e. human beings. This study however compares between 2 accepted means of alcohol intoxication namely interperitoneal (i.p) and oral (o.r) routes. A total of 24 Rats were divided into 4 groups vis-à-vis (a) oral group administered with 63mg (10%) and 130mg (20%) ethanol kg-1 body weight (chronic) for a period of 14 days and concomitantly, the intraperitoneal group, injected with 130mg (20%) ethanol kg-1 body weight for a period of 7 days (acute) and lastly the control group injected with normal saline as the body weight dictated. After this period of intoxication the following biochemical parameters were determined ALT, AST, Cholesterol and Electrolyte balance. Ethanol intoxicated rats (chronic and acute) recorded a significant increase in serum aspartate (AST) and Alanine (ALT) amino transferase activities, cholesterol, and a decrease in electrolyte (potassium) the elevation in total protein was not significant with the i.p group showing the highest elevations. Hence, aspartate (AST) and alanine (ALT) aminotransferase, cholesterol, electrolyte estimation (potassium) may be used as biomarkers for the early diagnosis of ethanol toxicity in human beings.
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    Open Access
    Ebuehi, O.A.T.,Erukainure, O.L.,Adeboyejo, F.O.,Elemo, G.N. Modulation of lipid peroxidation, hypolipidemic and antioxidant activities in brain tissues of diabetic rats by fibre - Enriched biscuits
    (Journal of Acute Disease, 2012) Ebuehi, O.A.T.; Erukainure, O.L.; Adeboyejo, F.O.; Elemo, G.N.
    Objective: To investigate the effect of feeding fibre - enriched biscuit on the antioxidant and hypolipidemic activities in brain tissues of diabetic rats. Method: Diabetes was induced by a single intraperitoneal injection of alloxan. Treatment lasted for 14 d, after which the rats were sacrificed by cervical dislocation. Brain tissues were used for the assessment of GSH, catalase, SOD and lipid peroxidation as well as lipid profiles. Result: Induction of diabetes led to a significant decrease in GSH level, elevated SOD and catalase activities. These were significantly modified by the biscuits. There was an elevated level of malondialdehyde in the brain tissues of the untreated diabetic rats; this was significantly reduced by the biscuits. There was a significant decrease in HDL and a significant increase in LDL levels, total cholesterol and triglycerides in the untreated (diabetic) rats. Feeding with fibre - enriched biscuits led to decrease in the levels of total cholesterol, triglyceride, LDL - cholesterol and caused a significant increase in the levels of HDL. Conclusions: These results suggest a therapeutic and protective effect of the fibre - enriched biscuits against diabetic - induced brain toxicity in rats.
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    Open Access
    Modulation of lipid peroxidation, hypolipidemic and antioxidant activities in brain tissues of diabetic rats by fibre - Enriched biscuits
    (Journal of Acute Disease, 2012) Ebuehi, O.A.T.; Erukainure, O.L.; Adeboyejo, F.O.; Elemo, G.N.
    Objective: To investigate the effect of feeding fibre - enriched biscuit on the antioxidant and hypolipidemic activities in brain tissues of diabetic rats. Method: Diabetes was induced by a single intraperitoneal injection of alloxan. Treatment lasted for 14 d, after which the rats were sacrificed by cervical dislocation. Brain tissues were used for the assessment of GSH, catalase, SOD and lipid peroxidation as well as lipid profiles. Result: Induction of diabetes led to a significant decrease in GSH level, elevated SOD and catalase activities. These were significantly modified by the biscuits. There was an elevated level of malondialdehyde in the brain tissues of the untreated diabetic rats; this was significantly reduced by the biscuits. There was a significant decrease in HDL and a significant increase in LDL levels, total cholesterol and triglycerides in the untreated (diabetic) rats. Feeding with fibre - enriched biscuits led to decrease in the levels of total cholesterol, triglyceride, LDL - cholesterol and caused a significant increase in the levels of HDL. Conclusions: These results suggest a therapeutic and protective effect of the fibre - enriched biscuits against diabetic - induced brain toxicity in rats.
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    Open Access
    Nicotinamide Ameliorates Serum C-Peptide and Brain Tryptophan Levels in STZ-Induced Diabetic Sprague- Dawley Rats .
    (International Journal of Science and Research (IJSR), 2015) Ebuehi, O.A.T.; Fregene, C; Ihegboro, G.O.
    Metabolic complications of Type-1 diabetes mellitus are a significant source of morbidity and mortality. Nicotinamide treatment has been reported to reverse pancreatic beta cell damage and used to prevent Type-1 diabetes mellitus. The effects of nicotinamide on fasting blood glucose, serum C-peptide and brain tryptophan have not been fully investigated. The objective of the study is to evaluate the effect of nicotinamide on fasting blood glucose, serum C-peptide and brain tryptophan levels in Streptozotocin (STZ)- induced diabetic rats. Type-1 diabetes was induced by intra-peritoneal injection of streptozotocin (55mg/kg) using Sprague-Dawley rats. After 2 days, the rats were divided into diabetic control and nicotinamide-treatment group. Nicotinamide was orally administered at daily doses of 375mg/kg and 500mg/kg for a period of 4 weeks, while another group of rats without this treatment served as control. The diabetic control group showed significant (p < 0.05) increase in fasting blood glucose, but a decrease in serum C–Peptide and brain tryptophan levels compared with the control. Treatments with 375mg/kg and 500mg/kg nicotinamide showed a significant decrease in fasting blood glucose, but an increase in serum C-peptide and brain tryptophan levels compared with diabetic control. Data of the study indicate that nicotinamide may prevent diabetic complications by alleviating its metabolic symptoms of hyperglycemia and polyphagia, which may ameliorates pancreatic islet cell damage in diabetic rats.
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    Open Access
    Phytochemical Investigation and the Effects of Aqueous Plant Extracts of Viscum Album on Antioxidant Property and Biochemical Profile as a Measure of its Therapeutic Value
    (International Journal of Science and Research (IJSR), 2014) Ebuehi, O.A.T.; Ihegboro, G.O.
    The aim of the research was to investigate the phytochemical compounds inherent in the aqueous plant extracts of Viscum album, its antioxidant property and the effects on biochemical parameters in wistar rats. Phytochemical investigation revealed the presence of alkaloid, flavonoid, saponin, tannin, carbohydrate, reducing sugar, protein, cardiac glycoside and steroidal aglycon in both the leaf and stem extracts, although the stem extract showed higher concentration of the phytochemical compounds compared to that of the leaf extract. The plant extracts possessed antioxidant properties using the DPPH free radical scavenging method. Biochemical results showed significant reduction in the concentrations of liver enzyme markers such as Alanine transaminase (ALT), Aspartate transaminase (AST) and Alkaline Phosphatase (ALP) suggesting that the aqueous plant extracts possessed hepatoprotective property. There were also significant reduction in the levels of glucose, cholesterol, Triglyceride, Total protein, Albumin, Creatinine and Urea.