Department Of Microbiology
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The first phase in the development of the University began with the establishment of the Faculty of Science in October 1964. In October 1967, the Faculty was split into two schools i.e., the School of Biological Sciences, and the School of Mathematical and Physical Sciences.
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Browsing Department Of Microbiology by Author "Adekunle, A.A."
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- ItemOpen AccessBiodegradation and detoxification of Scarlet RR dye by a newly isolated filamentous fungus, Peyronellaea prosopidis(Elsevier, 2018-03) Bankole, P.O.; Adekunle, A.A.; Obidi, O.F.; Chandanshive, V.V.; Govindwar, S.P.Efficient mitigation and management of environmental pollution caused by indiscriminate disposal of textile industry dyes and effluents deserves special attention. The aim of the present study was to evaluate the efficiency of Peyronellaea prosopidis for the decolorization, degradation and detoxification of Scarlet RR dye. Ultraviolet visible spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, High-Performance Liquid Chromatography and Gas ChromatographyeMass Spectrometry (GCeMS) were used in analyzing the degraded metabolites of the dye. P. prosopidis showed decolorization potency on Scarlet RR dye, dye mixture and textile industry dye effluent at a concentration of 10 mg L 1 and up to 90, 84 and 85% within 5 d. Maximum decolorization of Scarlet RR dye (10 mg L 1) by P. prosopidis was achieved at pH 6, temperature (35 C) and biomass dose (1 g). Furthermore, 68, 88 and 91% reduction was recorded in the biological oxygen demand, chemical oxygen demand and color intensity of the textile industry effluent, respectively, after treatment with P. prosopidis. The degradation mechanism mediated by enzymes revealed significant inductions in lignin peroxidase (85%), laccase (58%), and manganese peroxidase (48%) after treatment of Scarlet RR dye with P. prosopidis. FTIR spectra of the metabolites showed significant disappearance and shifts in peaks in comparison with controls. Metabolites obtained from the GCeMS analysis were: N-(1l3-chlorinin-2-yl)-2-{methyl[(4-oxo-3,4-dihydroquinolin-2-yl)methyl]amino}acetamide; N- (1l3-chlorinin-2-yl)-2-{[(4-oxo-3,4-dihydronaphthalen-2 yl)methyl]amino}acetamide; 5-({[2-(1l3-chlorinin-2-ylamino) ethyl]amino}methyl)cyclohexa-2,4-dien-1-one and N-ethyl-1l3-chlorinin-2-amine after degradation of Scarlet RR dye. The detoxified status of the dye metabolites was confirmed with significant growth of plumule and radicle.
- ItemOpen AccessDegradation of indigo dye by a newly isolated yeast, Diutina rugosa from dye wastewater polluted soil(Elsevier, 2017-08) Bankole, P.O.; Adekunle, A.A.; Obidi, O.F.; Olukanni, O.D.Isolation, identification, and characterization of newly isolated yeast, Diutina rugosa capable of decolorizing indigo dye were investigated in this study. Molecular and phylogenetic analyses of 23S rRNA sequence data indicated that the yeast belonged to the new genus, Diutina. The optimization of physicochemical parameters such as pH of the solution (2–8), initial dye concentration (10–60 mg L−1), adsorbent mass (0.1–2 g), and temperature (10–50 °C) was studied to scale- up the conditions of dye removal. Furthermore, complete decolorization (99.97%) of indigo dye (10 mg L−1) was achieved at pH 2, temperature 30 °C and 2.0 g cell biomass within 5 days. Degradation was monitored through UV–vis spectrophotometric, FTIR, and GCMS analyses. The results of FTIR analysis obtained revealed the loss and shifts in spectra peaks of the experimental in comparison with the biological control. Possible degradation pathway was proposed using the intermediate metabolites; 1, 2-dihydro-3H-indol-3-one and cyclopentanone obtained through GCMS analysis. The enzyme analyses revealed significant inductions and major roles played by NADH-DCIP reductase and lignin peroxidase in the asymmetric cleavage, initial reduction and deamination of indigo dye. The equilibrium experimental data were fitted to Langmuir, Freundlich and Temkin adsorption isotherms with high adjusted coefficient of determination values; adjR2 = 0.907, adjR2 = 0.867, and adjR2 = 0.965 respectively. However, the Langmuir and Temkin adsorption isotherms affirmed the monolayer and heterogeneous biosorption characteristics of Diutina rugosa. Temkin adsorption isotherm model (R2 = 0.971) represented the best fit of experimental data than other isotherm models
- ItemOpen AccessMycodecolorization of Reactive Red HE7B dye by Achaetomium strumarium and Aspergillus flavus and shelf life determination(Cogent OA, 2017-01-01) Bankola, P.O.; Adekunle, A.A.; Obidi, O.F.The decolorization of Reactive Red HE7B dye, a sulphonated reactive azo dye, was achieved under static condition with Achaetomium strumarium (KR262716) and Aspergillus flavus (KJ880096). The growth profle of isolated organisms and physicochemical parameters from the dye was ftted into a multiple linear regression model to predict the shelf life. The dye has an estimated shelf life of 36 months. The fungi showed complete decolorization of over 95% within 15 days. While A. flavus achieved maximum decolorization at a neutral pH, maximum decolorization was achieved by A. strumarium at pH of 6. The fungi optimally decolorized the dye at a temperature of 35°C. The UV–Visible and FTIR spectrometric analyses were used to study decolorization. The decrease, shifts and disappearance of peaks in UV and FTIR spectra of treated samples indicated myco-decolorization. The formation of aromatic amine was supported by Fourier Transform Infrared spectrometry (FTIR), which revealed the disappearance of certain peaks, particularly those of the aromatic C–H bending at 600–800 cm−1. In phytotoxicity studies, A. strumarium showed higher detoxifcation efciency on Reactive Red HE7B dye than A. flavus. The results conclusively showed that A. strumarium exhibited greater decolorization efficiency on Reactive Red HE7B dye than A. flavus.