Oral and Maxillofacial Pathology/Biology - Scholarly Publications

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Browsing Oral and Maxillofacial Pathology/Biology - Scholarly Publications by Author "Odukoya, O"

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    Open Access
    Analysis of Elastic Tissue in Histological Variants of Pleomorphic Salivary Adenoma seen at the Lagos University Teaching Hospital over a period of 35 Years
    (West Africa Journal of Medicien, 2014-07) Effiom, OA; Olawuyi, AB; Olorunsola, KD; Olisa, AG; Odukoya, O; Ogundana, OM; Ajayi, OF; Odukoya, O
    BACKGROUND: Pleomorphic salivary adenoma (PSA), is known for its morphologic diversity. While reports of elastic tissue in PSA have been documented, the distribution of this tissue in histological variants of the tumour has not been documented. Perhaps such features may influence biological behaviour of these variants. OBJECTIVE: To classify PSA in our series into histological variants, and determine possible variation in elastic tissue distribution in them. METHODS: Eighty eight histologically diagnosed cases of PSA in the oral biopsy archives of the department of Oral Biology and Pathology, Lagos University Teaching Hospital, were retrieved. New H&E sections were cut to reconfirm diagnosis and Verhoeff-Van Gieson's stained sections were cut for demonstration of elastic tissue. Seifert et al.'s (1976) histological classification was applied and elastic tissue presence was determined and quantified for each case. Parameters studied included; sex, age, site, histological subtypes and presence of elastic tissue. Statistical analysis was undertaken using the EPI-INFO version 3.4. RESULTS: Male:female ratio was 1:1.3. Most cases (63.6%) occurred in the age group of 21-40 years. Generally, palate (42.0%) was the most commonly affected site, while 53.4% of cases were in the minor salivary glands. Seifert et al. classified subtype II lesions were the most frequently observed (39.7%) and elastic tissue was confirmed in 91.0% of cases. No association was noted between proportion of elastic tissue and histological variants. CONCLUSION: Seifert et al subtype II was the most frequently observed and no association was observed between proportion of elastic tissue and the histological variant of PSA
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    Open Access
    Enhanced basal autophagy supports ameloblastoma-derived cell survival and reactivation
    (Elsevier Ltd, 2018-11-14) Dhingra, A; Odukoya, O; Arotiba, G; Olawuyi, A; Boesze-Battaglia, K; Sharp, R.C; Efiom, O.A; Akintoye, S.O
    OBJECTIVES: Ameloblastoma is an aggressive odontogenic jaw neoplasm. Its unlimited growth confers high potential for malignant transformation and recurrence. It is unclear why ameloblastoma is highly recurrent despite surgical resection with a wide margin of normal tissue. While canonical autophagy can be used to degrade and eliminate damaged cellular components, it is also a protective mechanism that provides energy and vital metabolites for cell survival. We used ameloblastoma-derived cells to test the hypothesis that autophagic processes play a role in survival and reactivation of ameloblastoma. METHODS: Primary epithelial (EP-AMCs) and mesenchymal (MS-AMCs) ameloblastoma-derived cells were established from tissue samples of solid multicystic ameloblastoma. Clonogenic capacity and basal autophagic capacity were assessed in ameloblastoma-derived cells relative to human odontoma-derived cells (HODCs) and maxilla-mesenchymal stem cells (MX-MSCs). Ability of ameloblastoma-derived cells to survive and form new ameloblastoma was assessed in mouse tumor xenografts. RESULTS: EP-AMCs were highly clonogenic (p < 0.0001) and demonstrated enhanced basal levels of autophagic proteins microtubule-associated protein 1-light chain 3 (LC3) (p < 0.01), p62 (Sequestosome 1, SQSTM1) (p < 0.01), and the LC3-adapter, melanoregulin (MREG) (p < 0.05) relative to controls. EP-AMCs xenografts regenerated solid ameloblastoma-like tumor with histological features of columnar ameloblast-like cells, loose stellate reticulum-like cells and regions of cystic degeneration characteristic of follicular variant of solid multicystic ameloblastoma. The xenografts also displayed stromal epithelial invaginations strongly reactive to LC3 and p62 suggestive of epithelial-mesenchymal transition and neoplastic odontogenic epithelium. CONCLUSIONS: EP-AMCs exhibit altered autophagic processes that can support survival and recurrence of post-surgical ameloblastoma cells.OBJECTIVES: Ameloblastoma is an aggressive odontogenic jaw neoplasm. Its unlimited growth confers high potential for malignant transformation and recurrence. It is unclear why ameloblastoma is highly recurrent despite surgical resection with a wide margin of normal tissue. While canonical autophagy can be used to degrade and eliminate damaged cellular components, it is also a protective mechanism that provides energy and vital metabolites for cell survival. We used ameloblastoma-derived cells to test the hypothesis that autophagic processes play a role in survival and reactivation of ameloblastoma. METHODS: Primary epithelial (EP-AMCs) and mesenchymal (MS-AMCs) ameloblastoma-derived cells were established from tissue samples of solid multicystic ameloblastoma. Clonogenic capacity and basal autophagic capacity were assessed in ameloblastoma-derived cells relative to human odontoma-derived cells (HODCs) and maxilla-mesenchymal stem cells (MX-MSCs). Ability of ameloblastoma-derived cells to survive and form new ameloblastoma was assessed in mouse tumor xenografts. RESULTS: EP-AMCs were highly clonogenic (p < 0.0001) and demonstrated enhanced basal levels of autophagic proteins microtubule-associated protein 1-light chain 3 (LC3) (p < 0.01), p62 (Sequestosome 1, SQSTM1) (p < 0.01), and the LC3-adapter, melanoregulin (MREG) (p < 0.05) relative to controls. EP-AMCs xenografts regenerated solid ameloblastoma-like tumor with histological features of columnar ameloblast-like cells, loose stellate reticulum-like cells and regions of cystic degeneration characteristic of follicular variant of solid multicystic ameloblastoma. The xenografts also displayed stromal epithelial invaginations strongly reactive to LC3 and p62 suggestive of epithelial-mesenchymal transition and neoplastic odontogenic epithelium. CONCLUSIONS: EP-AMCs exhibit altered autophagic processes that can support survival and recurrence of post-surgical ameloblastoma cells.
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    Open Access
    Epithelial jaw cysts: analysis of 126 Nigerian cases
    (African Dental Journal, 1996) Ogunlewe, MO; Odukoya, O; Akinwande, JA
    One hundred and twenty-six Nigerian cases of epithelial jaw cysts were retrieved from case notes and biopsy records of the Department of Oral and Maxillofacial Surgery and Department of Oral Pathology and Oral Biology of the Lagos University Teaching Hospital. The cases were analysed for age, sex, site and methods of management. Results show that developmental odontogenic cysts (57.14%) were more common than inflammatory odontogenic cysts (26.94%). Dentigerous cyst (22.22%) was the most common epithelial jaw cyst, followed by radicular cyst (21.43%). Developmental non-odontogenic cysts were more than twice as common in females as in males. Sixty-eight per cent of epithelial jaw cysts were treated by enucleation, 10% by marsupialisation, 13% by jaw resection and 9% by surgical excision.
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    Open Access
    Nucleolar organizer regions in jaw tumours of cartilaginous origin
    (West African Journal Of Medicine, 2001-03) Afolabi, OC; Odukoya, O; Arole, G; Banjo, A F
    Nucleolar organizer regions [NORs] are loops of DNA that transcribe to ribosomal RNA. They can be visualized as intranuclear black dots by histochemical staining with a colloid silver solution. Silver-stained nucleolar proteins [AgNORs] were counted in cases comprising of primary chondrosarcomas of three histologic grades, in chondromyxoid fibroma and in controls comprising of normal bone and cartilage tissues of the jaw bones. The AgNOR counts increased step-wisely from normal bone tissue [1.11 0.4], chondromyxoid fibroma [2.66 0.78], grade I chondrosarcoma [3.94 0.34], grade II chondrosarcoma [4.32 0.52], and grade III chondrosarcoma [5.54 0.44]. There was a statistically significant difference in the mean AgNOR counts between grade 1 and grade III chondrosarcoma [p < 0.05]. The mean AgNOR counts for benign cartilaginous [chondromyxoid fibroma] tumour was significantly lower than the mean, AgNOR count for malignant cartilaginous tumours [chondrosarcomas] [p < 0.05]. The results in the present study indicate that silver colloid staining is a useful technique for evaluating the proliferative activity of chondrosarcoma and benign cartilaginous tumour such as chondromyxoid fibroma.
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    Open Access
    Pattern of distribution of odontogenic tumours in sub-Saharan Africa
    (John Wiley & Sons A/S, 2017) Ogundana, OM; Effiom, OA; Odukoya, O
    Objective: To analyse documented reports on odontogenic tumours (OTs) in sub-Saharan Black Africa and to create awareness of the need for more reports on this topic. Methods: There were 2,186 African cases of OTs presented at the International Association of Oral and Maxillofacial Pathologists (IAOP) Congress (Cape Town, South Africa) in 1998 that were added to 2,198 cases derived from reports published in the scientific literature from 1998 to date. The analysis undertaken included frequency distribution of available data on OTs that was listed in the 2005 World Health Organization (WHO) classification. The chi-square test was applied as appropriate. Results: A total of 4,384 cases of OTs from only 14 of 47 sub-Saharan Black African countries were analysed. Ameloblastoma was the OT most commonly reported, accounting for 74.2% of OTs (and 86.9% of category A OTs), followed by keratocystic odontogenic tumour (KCOT) (6.9%) and odontogenic myxoma (ODM) (4.9%). Malignant OTs were reported at a low frequency, of 1.4%. Nigeria contributed 38.7% of the cases of OT in this series. Conclusions: Ameloblastoma is the commonest OT in sub-Saharan Africa and has a higher occurrence in male than in female subjects. With data on OTs not available from 33 of 47 sub- Saharan African countries, additional reports are needed.
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    Open Access
    Salivary Adenoid Cystic Carcinoma: Clinicohistologic Study in a Nigerian Tertiary Institution
    (Wolters Kluwer - Medknow, 2019) Ajayi, OF; Ogundana, OM; Akinshipo, AO; Olawuyi, AB; Odukoya, O
    Background: Adenoid cystic carcinoma (ADCC) is a slow‑growing salivary gland tumor with high recurrence and mortality rates. Histologic variants present variable aggression. This has not been investigated in Nigeria. This study aimed to investigate association of histologic variants with clinical aggression in Nigerian cases. Patients and Methods: Fifty‑nine ADCC from 363 salivary gland tumors were selected from the departmental oral biopsy archives. Clinical data were retrieved, and hematoxylin and eosin sections were reviewed for confirmation and categorization into solid, cribriform, and tubular (modified Perzin, Spiro and van Weert systems). Estimated mean tumor growth rates (EMTGRs) were computed and matched with histologic variants. Statistical analysis was Chi‑square, Kruskal–Wallis, and Mann–Whitney’s test. P value was ≤0.05. Statistical package was SPSS. Results: Age ranged between 7 and 83 years (mean 49.2 ± 16.8 years). About 75.1% occurred in the 4th–6th decade (P = 0.02). Most common histologic variant was predominantly cribriform no solid (PCNS) pattern (40.7%). In major salivary glands, there was association between histologic variant and EMTGR (P = 0.025). PCNS had the highest EMTGR (0.840) followed by predominantly solid (PS) (EMTGR, 0.744). These were significantly higher than predominantly tubular no solid (PTNS) (EMTGR, 0.442) and predominantly tubular 30% solid (EMTGR, 0.115). In minor glands, there was also association between histologic variants and EMTGR (P = 0.017). However, the highest EMTGR (0.509) occurred in PTNS followed by PCNS (0.428). These were significantly higher than PS (0.259) with the least EMTGR. Conclusion: Trend of clinical aggression of histological variants based on EMTGR of ADCC varies depending on the type of salivary gland (major vs. minor).