Department of Haematology and Blood Transfusion

Permanent URI for this community

https://ir.unilag.edu.ng/handle/123456789/3606

Browse

Browsing Department of Haematology and Blood Transfusion by Subject "Anaemia"

Now showing 1 - 6 of 6
  • Item
    Open Access
    Cord blood haemoglobin and ferritin concentrations in newborns of anaemic and non-anaemic mothers in Lagos, Nigeria
    (Nigerian Medical Journal, 2013-02) Adediran, A; Gbadegesin, A; Adeyemo, TA; Akinbami, A; Osunkalu, VO; Ogbenna, A; Akanmu, AS
    Background: Pre-delivery haemoglobin and serum ferritin concentrations of anaemic and non-anaemic mothers were determined, and cord blood haemoglobin and serum ferritin concentrations of their newborns were compared. This is to establish the mean values for pre-delivery haemoglobin and serum ferritin concentrations of anaemic and non-anaemic mothers and the cord blood haemoglobin and serum ferritin concentrations of their newborns at term. Materials and Methods: A case–control study was done involving 142 pregnant women and their newborns. They were divided into two groups - the anaemic group (n = 65) and the non-anaemic (n = 77) group. Five millilitres of blood was collected from each woman and 2 ml was collected from the cord of their newborns into ethylenediaminetetraacetic acid (EDTA) bottle and plain bottle for full blood count analysis and ferritin assay, respectively. Results: The mean pre-delivery haemoglobin concentrations of the women in anaemic group and non-anaemic group were 9.5 ± 1.01 g/dl and 12.15 ± 1.07 g/dl, respectively, and their mean serum ferritin concentrations were 64.45 ± 138.76 μg/l and 32.83 ± 35.36 μg/l, respectively. The mean cord blood haemoglobin concentrations for anaemic and for non-anaemic groups were 12.54 ± 2.54 g/dl and 13.44 ± 2.23 g/dl (P = 0.02), respectively, and the mean cord blood serum ferritin concentrations (non-anaemic, 69.38 ± 78.88 μg/l; anaemic, 7.26 ± 115.60 μg/l) (P = 0.00) were higher in the newborns of non-anaemic than of anaemic mothers. Significant association was found between maternal anaemia and cord blood ferritin concentrations (P = 0.025). Conclusion: Maternal anaemia has significant effects on cord blood haemoglobin and serum ferritin concentrations.
  • Item
    Open Access
    Cord blood haemoglobin and ferritin concentrations in newborns of anaemic and non‐anaemic mothers in Lagos, Nigeria
    (Nigerian Medical Journal, 2013) Adediran, A; Gbadegesin, A; Adeyemo, T.A; Akinbami, A; Osunkalu, V; Ogbenna, A.A; Akanmu, A.S
    Background: Pre‐delivery haemoglobin and serum ferritin concentrations of anaemic and non‐anaemic mothers were determined, and cord blood haemoglobin and serum ferritin concentrations of their newborns were compared. This is to establish the mean values for pre‐delivery haemoglobin and serum ferritin concentrations of anaemic and non‐anaemic mothers and the cord blood haemoglobin and serum ferritin concentrations of their newborns at term. Materials and Methods: A case–control study was done involving 142 pregnant women and their newborns. They were divided into two groups – the anaemic group (n = 65) and the non‐anaemic (n = 77) group. Five millilitres of blood was collected from each woman and 2 ml was collected from the cord of their newborns into ethylenediaminetetraacetic acid (EDTA) bottle and plain bottle for full blood count analysis and ferritin assay, respectively. Results: The mean pre‐delivery haemoglobin concentrations of the women in anaemic group and non‐anaemic group were 9.5 ± 1.01 g/dl and 12.15 ± 1.07 g/dl, respectively, and their mean serum ferritin concentrations were 64.45 ± 138.76 μg/l and 32.83 ± 35.36 μg/l, respectively. The mean cord blood haemoglobin concentrations for anaemic and for non‐anaemic groups were 12.54 ± 2.54 g/dl and 13.44 ± 2.23 g/dl (P = 0.02), respectively, and the mean cord blood serum ferritin concentrations (non‐anaemic, 69.38 ± 78.88 μg/l; anaemic, 7.26 ± 115.60 μg/l)(P = 0.00) were higher in the newborns of non‐anaemic than of anaemic mothers. Significant association was found between maternal anaemia and cord blood ferritin concentrations (P = 0.025). Conclusion: Maternal anaemia has significant effects on cord blood haemoglobin and serum ferritin concentrations.
  • Item
    Open Access
    Haemoglobin and ferritin concentrations of pregnant women at term
    (Obstetric Medicine, 2011) Adediran, A; Gbadegesin, A; Adeyemo, T.A; Akinbami, A.A; Akanmu, A.S; Osunkalu, V; Ogbenna, A.A; Oremosu, A
    Background: Anaemia in pregnancy is defined as haemoglobin (Hb) concentrations of less than 11 g/dL while low ferritin is defined as serum ferritin (SR) levels of less than 10 mg/L. Hb and ferritin concentrations of pregnant women at term were determined to establish their mean values and to determine the prevalence of anaemia in our locality. Methods: Haemoglobin and ferritin levels of 170 non-smoking and HIV-negative pregnant women were determined at term. The majority 143 of 170 (84.1%) of the pregnant women recruited for the study, booked at the beginning of the second trimester and received 200 mg elemental iron in three divided doses and 5 mg folic acid daily which were commenced at booking. Five millilitres of blood were collected from each patient at term into EDTA bottles for full blood count analysis and another 5 mL into plain bottles for SR assay. Results: The mean Hb and ferritin values were 10.9+1.9 and 47.84+98.39 mg/L, respectively. The prevalence of anaemia at term was 46.4%. Only 11.2% (19 of 170) of pregnant women at term had low SR (iron stores). A statistically significant relationship was found between women’s education and SR (P 1⁄4 0.032). Booking status also correlated directly with SR and haemoglobin concentrations, while increasing age and parity did not. Conclusion: About half of the patients were anaemic. Iron deficiency is not the major cause of anaemia in pregnancy in this study because the majority of the pregnant women had normal iron stores. Education and booking status are possible factors that con- tribute to anaemia.
  • Item
    Open Access
    Impact of HIV Infection and Zidovudine Therapy on RBC Parameters and Urine Methylmalonic Acid Levels
    (Interdisciplinary Perspectives on Infectious Diseases, 2016-01-13) Adediran, A; Osunkalu, VO; Wakama, T; Olabode, S; Akinbami, A; Ebele, U; A, AS
    Background. Anaemia is a common complication of human immunodeficiency virus (HIV) infection. The aim of this study was to investigate the impact of HIV infection and zidovudine on red blood cells (RBC) parameters and urine methylmalonic acid (UMMA) levels in patients with HIV infection. Material and Methods. A cross-sectional study involving 114 subjects, 94 of which are HIV-infected nonanaemic and 20 HIV negative subjects (Cg) as control. Full blood count parameters and urine methylmalonic acid (UMMA) level of each subject were determined. Associations were determined by Chi-square test and logistic regression statistics where appropriate. Results. Subjects on zidovudine-based ART had mean MCV (93 fL) higher than that of control group (82.9 fL) and ART-naïve (85.9 fL) subjects and the highest mean RDW. Mean UMMA level, which reflects vitamin B12 level status, was high in all HIV-infected groups but was significantly higher in ART-naïve subjects than in ART-experienced subjects. Conclusion. Although non-zidovudine therapy may be associated with macrocytosis (MCV > 95 fL), zidovudine therapy and ART naivety may not. Suboptimal level of vitamin B12 as measured by high UMMA though highest in ART-naïve subjects was common in all HIV-infected subjects.
  • Item
    Open Access
    Impaired Appropriateness of Erythropoietin in Anaemic HIV Infected Patients
    (International Blood Research and Reviews, 2016) Olu-Taiwo, A; Amusu, A.O; Olatunya, O.S; Adediran, A; Ajayi, E.A; Ogbenna, A.A; Akanmu, A.S
    This study was carried out to determine the degree of erythropoietin response in anaemic HIV infected persons. Methods: A total of 120 subjects were studied. These comprised of the study group made up of 40 HIV infected treatment naive patients who had anaemia with Haemoglobin level <10g/dL and 80 control subjects who were age and sex matched. The controls consisted of 20 HIV infected non￾anaemic individuals, 40 non-HIV infected anaemic individuals, and 20 non-HIV infected non￾anaemic individuals. Blood samples were collected for haemoglobin estimation, white cell count and platelet count by automated counter. The CD4+ cell count was done by semi-automated flow cytometer. The viral load was quantitated using PCR- based diagnostic tests and serum erythropoietin level was estimated using ELISA technique. All patients gave written informed consent with ethical approval by the hospital ethics and research committee. Results: The study groups consisted of 40 males and 80 females. The mean age of male HIV anaemic subjects (45.15±5.63, N=13) was significantly higher than that of the female HIV anaemic subjects (34.16±7.21, N=25) p=0.005. The mean CD4+ cell count of HIV non-anaemic subjects (530.55 cells/µL ±423.35) was significantly higher than HIV anaemic subjects (188.18 cells/µL ±157.09) (p=0.0009). Using regression equation the expected serum erythropoietin values for a given haemoglobin level in HIV subjects was estimated. The appropriateness of erythropoietin level was then determined and a ratio of <0.8 was considered inappropriate. Seventy one percent of the HIV anaemic subjects had erythropoietin response ratio less than 0.8. Conclusion: There was a blunted erythropoietin response for the degree of anaemia in HIV compared with HIV negative subjects.
  • Item
    Open Access
    Morphologic Evaluation of Anemia – I
    (Biology and Medicine, 2016) Adewoyin, S.A; Ogbenna, A.A
    Anaemia is a feature of many tropical diseases. Anaemia diagnosis therefore remains a crucial intervention among physicians in developing countries. A barrage of laboratory test (anaemic work-up) is usually deployed in differentiating its underlying cause. However, central to anaemia evaluation is the morphology of the red cells and other cell lines. Conventionally, initial laboratory tests include full blood count, reticulocyte count and peripheral blood film (PBF). PBF is often a clinical request, performed by skilled technologist and reported by haematologist/ haematomorphologist. Findings from PBF are reviewed and reported in the light of patient’s clinical history and examination findings. This article therefore aims to promote PBF evaluation in anaemic patients, facilitate laboratory communication of morphologic findings among clinicians, particularly in developing nations where advanced investigations such as flow cytometry and molecular diagnosis may not be readily available, invariably improving patient care and treatment outcomes.