Genetic Diversity of Vernonia as Revealed By Random Amplified Polymorphic DNA (RAPD) Markers
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Date
2018-04
Authors
Nwakanma, N.M.C.
Oboh, B.O.
Adekoya, K.O.
Ogunkanmi, L.A.
Journal Title
Journal ISSN
Volume Title
Publisher
Egyptian Academic Journal of Biological Sciences (H. Botany)
Abstract
Vernonia Schreb. is a genus in the family Asteraceae. It has over
1000 species which may be trees, shrubs, woody climbers or herbs.
Some of the species are economically important as sources of food and
herbal medicine as well as for industrial purposes. This study was
carried out to assess the genetic diversity of Vernonia species in
Nigeria. Molecular characterization was carried out on a total of 50
Vernonia samples consisting of 34 samples (13 species) from field
collections and 16 samples (10 species) from Ife Herbarium collections
making a total of 23 species using 4 RAPD primers. Analysis of the
molecular data was done using NTsys version 2.02j computer
programme. Results from molecular studies showed amplification
through polymerase chain reaction (PCR) with 4 RAPD markers
namely OPA-02, OPD-14, OPC-10 and OPE-01. Analysis of the
molecular data grouped the samples into 4 Groups with only one
species ungrouped. Group I had 17 species, II had 3 species, III had 6
species while IV had 2 species. V. amygdalina was represented in all 4
Groups, V. glabra and V. guineense were present in Groups 1 and 3, V.
tenoreana and V. purpurea were in Groups 1 and 2 respectively, while
V. colorata remained ungrouped. All 50 Vernonia samples used in the
molecular study clustered at 64% and analyzed at a similarity
coefficient of 72%. This analysis revealed that RADP markers are
useful tools in assessing the level of genetic diversity in Vernonia.
Description
Keywords
Genetic Diversity , Molecular Characterization , PCR , RAPD , Vernonia
Citation
Nwakanma, N. M. C., Oboh, B.O., Adekoya, K.O., and Ogunkanmi, L. A. (2018). Genetic Diversity of Vernonia as Revealed By Random Amplified Polymorphic DNA (RAPD) Markers. Egyptian Academic Journal of Biological Sciences H. Botany. 9 (1) : 27 - 38