Identification and Characterization of Bacteria Isolated from Pipe-borne Water Samples in Selected Areas of Lagos Using Api kits and RAPD-PCR
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Date
2017-05
Authors
Adesanya, K.M.
Adekoya, K.O.
Ogunkanmi, L.A.
Oboh, B.O.
Journal Title
Journal ISSN
Volume Title
Publisher
Egyptian Academic Journal of Biological Sciences (G. Microbiology)
Abstract
Pipe-borne water is a major source of drinking water and contamination
of such water source could lead to an epidemic. The aim of this present study
is to isolate, identify and characterize microorganisms in pipe-borne water in
different locations of Lagos metropolis. Six microorganisms were isolated
from water samples collected at five locations. The isolates were subjected to
morphological as well as biochemical tests using gram staining and Analytical
Profile Index (API) tests respectively. Three primers (S gene, RAPD 1 and
RAPD 2) were used for the PCR amplification of DNA samples extracted
from the isolates and were identified as bacterial species: Bacillus polymyxa,
Bacillus alvei, Bacillus mycoides, Pasteurella aerogenes, Pasteurella
pneumotropica and Staphylococcus aureus. Gel electrophoresis of the
amplified DNA samples generated a total of 128 bands which were subjected
to statistical analysis using the NTSYS software (version 2.02j). The results as
shown in the UPGMA dendrograms revealed a similarity coefficient of 0.66
among B. polymyxa, B. alvei and B. mycoides as well as a high similarity
coefficient of 0.72 between P. pneumotropica and S. aureus. The presence of
spore-forming bacteria, B. mycoides B. polymyxa and B. alvei is considered a
biohazard as they can survive for long periods while the presence of P.
aerogenes, P. pneumotropica and S. aureus, known as opportunistic
pathogens is equally hazardous and can be a potential cause of water borne
disease. This present study, using RAPD-PCR technique has improved the
identification and characterization of microorganisms isolated from pipeborne water.
Description
Keywords
Pipe-borne water , Gel electrophoresis , RAPD , PCR , Bacteria
Citation
Adesanya, K.M., Adekoya, K.O., Ogunkanmi, L.A. and Oboh, B.O. (2017). Identification and Characterization of Bacteria Isolated From Pipe-Borne Water Samples In Selected Areas of Lagos Using API Kits and RAPD-PCR. Egyptian Academic Journal Of Biological Sciences (G. Microbiology) 9(1): 89 – 96.