Antinociceptive and anti-inflammatory Properties of Hydroethanolic Seed Extract of Monodora myristica (Annonaceae) in Rodents
Date
2016
Authors
Ishola, Ismail
Ikumawoyi, Victor
Afolayan, Gbenga
Oshoname, J. Olorife
Journal Title
Journal ISSN
Volume Title
Publisher
West African Journal of Pharmacy
Abstract
Background: Monodora myristica (Gaertn) Dunal (Annonaceae) is used in traditional medicine for cough,
rheumatism, hemorrhoids, diabetes, anemia and headaches.
Objective: This study was carried out to investigate the antinociceptive and anti-inflammatory effects of the
hydroethanolic seeds extract of Monodora myristca (HMM) in rodents.
Methods: HMM (50-200 mg/kg, p.o.) was administered 1 h before intraperitoneal or intraplantar injection of
V 0.6% /v acetic acid or 1% formalin (20 μL), respectively, to evaluate the antinociceptive property. Acute and
chronic anti-inflammatory effect was investigated using carrageenan-induced paw and xylene-induced ear
oedema, and cotton-pellet induced granuloma tests, respectively.
Results: HMM (50-200 mg/kg) produced dose dependent and significant decrease in mean number of
writhes in acetic acid-induced nociception and increased pain threshold to neurogenic and inflammatory
pain with 48.59 and 34.2% inhibition, respectively, in the formalin-induced nociception assay. The HMMinduced
antinociception was completely blocked by pre-treatment of mice with naloxone, p-
Chlorophenyalanine (serotonin synthase inhibitor; 100 mg/kg, i.p.) and sulpiride (D receptor antagonist; 2
50mg/kg) whereas glibenclamide (K sensitive channels blocker; 10 mg/kg) failed to reverse the ATP
antinociceptive effect of the extract. In acute inflammatory model, HMM produced time course inhibition of
carrageenan-induced paw oedema. In addition, pretreatment of mice with HMM inhibited xylene-induced
ear oedema by 60% comparatively similar to the effect of dexamethasone (83.90%). Moreover, in the cottonpellet
granuloma pouch, HMM (200 mg/kg) reduced granuloma formation by 52%.
Conclusion: The hydro-ethanolic seed extract of M. myristica possesses antinociceptive effect mediated
through interaction with opioidergic, serotonergic and dopaminergic systems and an anti-inflammatory
action through inhibition of inflammatory mediator's release. Finally, the study established the scientific basis
for its use in the management of pain and inflammatory conditions in traditional medicine.
Description
Keywords
Anti-nociceptive , Anti-inflammatory , Opioids , Serotonergic , Dopaminergic , Monodora myristica
Citation
Ismail O. Ishola, Victor O. Ikumawoyi, Gbenga O. Afolayan, Oshoname J. Olorife (2016) Antinociceptive and anti-inflammatory Properties of Hydroethanolic Seed Extract of Monodora myristica (Annonaceae) in Rodents West African Journal of Pharmacy 27 (1) 22-32