Application of a New Iterative Method To Analysis of Kinetics of Thermal Inactivation of Enzyme
In this paper, a new iterative method proposed by Temimi and Ansari is applied to analyze the kinetic of thermal inactivation of jack bean urease (EC220.127.116.11). The analyzed kinetics or reaction mechanisms consist of three-reaction steps and included the Arrhenius equation for the temperature dependence of rate constants as well as the temperature change in the initial heating period. The obtained solutions are used to study the model parameters on the kinetics of thermal inactivation of enzyme. The analytical solutions are verified with numerical solutions using Runge – Kutta with shooting method and good agreements are established between the solutions. The information given in this theoretical investigation will assist in the kinetic analysis of the experimental results over handling rate constants and molar concentrations.