Analysis of Antibiotics in poultry wastewater and droppings using solid phase extraction and High Performance Liquid Chromatography
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Date
2016
Authors
Oluseyi, T.O.
Oyeyiola, A.O.
Rabiu, B.
Mbadiwe, N.
Olayinka, K.O.
Silva, B.O.
Journal Title
Journal ISSN
Volume Title
Publisher
Chemical Society of Nigeria
Abstract
A method was developed for the extraction, pre-concentration and analysis of two antibiotics Amoxicillin (AMOX) and Oxytetracycline (OXY)) in poultry waste water and droppings. The method was based on solid phase extraction (SPE) and high performance liquid chromatography with ultraviolet detection (HPLC-UV). Optimization of the chromatographic conditions and the conditioning of the SPE sorbents were carried out to obtain the optimal condition for the analysis. Three eluting solvents were tested to establish the optimum conditions for the SPE procedure and pH values of samples were varied prior to HPLC analysis. Methanol gave the best percentage recoveries of 67.61% and 30.98%, followed by acetone with recoveries of 66.32% and 24.31% while acetonitrile had the poorest percentage recoveries of 64.23% and 19.05% for Amoxicillin and Oxytetracycline respectively. In the analysis of poultry waste water and droppings, the matrix components in the samples reduced the extraction efficiency leading to a decrease in the percentage recoveries of samples. The decrease in percentage recoveries obtained for the poultry waste water samples followed the same trend as the standard with methanol giving the best percentage recoveries of 60.27% and 23.04%, followed by acetone with recoveries of 45.37% and 21.65% while acetonitrile gave the poorest percentage recoveries of 29.12% and 16.21% for Amoxicillin and Oxytetracycline respectively.
Description
Staff Publications
Keywords
Amoxicillin , HPLC , Oxytetracycline , poultry waste , SPE , Research Subject Categories::NATURAL SCIENCES::Chemistry
Citation
Oluseyi, T. O., Et...al] (2016). Analysis of Antibiotics in poultry wastewater and droppings using solid phase extraction and High Performance Liquid Chromatography. Journal of Chemical Society of Nigeria, Vol.41(1).