Bacillus sphaericus a case study for antibacterial and photocatalytic effect of titanium nanoparticles and microcrystalline TIO2 on gram-positive paint microbes.
No Thumbnail Available
Date
2016
Authors
Obidi, O.F.
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Industrial Research and Technology
Abstract
Currently, there is a massive drift in bacteria as a result of emergence of resistant strains to conventional paint biocides. This study focused on a photodestructive strategy of paint microorganisms using titanium dioxide nanoparticles. The comparative toxicity of microcrystalline TiO2 and TiO2 Nanoparticles (NPs) on Bacillus sphaericus, a spore forming and environmentally diverse microbe isolated from spoilt paints was investigated. The antibacterial performance of the TiO2 NPs with 38nm particle size in the inhibition of Bacillus sphaericus and their toxic effect on the viability of the organism are illustrated and discussed in comparison with results obtained from the microcrystalline TiO2. The results show 90% reduction of the B. sphaericus cell viability at 10g/L concentrations of TiO2NPs under fluorescent light (400-700 nm) after 60 min irradiation. In contrast, a 40% reduction was observed for microcrystalline TiO2 (as determined by plate count assay) and scanning electron microscopy (SEM). In particular, the toxicity of both microcrystalline TiO2 and TiO2 NPs was also observed in the dark although with reduced influence. TiO2 NPs were found to exhibit bacteriostatic and photocatalytic effect on B. sphaericus as exposure time increases. Results of this study suggests that a longer exposure time could impact bactericidal activity. The results of the study showed that photocatalytic inactivation of paint bacteria can be directly linked to time of exposure.
Description
Staff publications
Keywords
Microcrystalline TiO2 , TiO Nanoparticles , Antibacterial agent , Bacillus sphaericus , Photokilling phenomenon , Research Subject Categories::NATURAL SCIENCES::Biology::Organism biology::Microbiology
Citation
Obidi, O.F. (2016). Bacillus sphaericus a case study for antibacterial and photocatalytic effect of titanium nanoparticles and microcrystalline TIO2 on gram-positive paint microbes. Journal of Industrial Research and Technology, Vol.5(1), 49-58pp.