Inorganic Nutrient Ion (PO43-, NO-3 and SO42-) as Essential Requirements in Bioremediation of soils polluted with Crude Petroleum
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Date
2016
Authors
Obidi, O.F.
Alo, M.N.
Nwachukwu, S.C.U.
Aboaba, O.O.
Journal Title
Journal ISSN
Volume Title
Publisher
Nigerian Journal of Microbiology
Abstract
The recovery rate of oil impacted agricultural soils by joint application of biostimulation agents such as PO43- and NO-3 (inorganic nutrient ions) and Bacillus subtilis (BS) as a bioaugmentation agent was investigated for 12 weeks. The dissolved oxygen (DO), pH, inorganic nutrient ions by ion chromatography and residual oil content by a gravimetric method, were determined over the period, as well as the microbial population density (MPD) by standard plate count (SPC). MPD enumerated for both the experimental (EDS) and the control (CDS) between weeks 2 and 7 showed increasing trends with the corresponding decreases in the oil content, particularly in EDS inoculated with BS. However, at week 8, PO43- and NO-3 depleted appreciably from 346 and 807 to 24 and 375 ppm respectively and simultaneously with a rapid decrease in MPD particularly the hydrocarbon utilizers (HCU). This apparently indicated the importance of nutrient ions in the bioremediation of oil polluted environments. The pH and DO values consistently decreased in both EDS and CDS over period. The result of this study showed that biostimulationinvolving inorganic nutrients adjustment and bioaugmentation by application of BS were the two key factors responsible for the remarkable differences in the results obtained for EDS particularly in the rapid oil content reduction when comared with CDS
Description
Staff publications
Keywords
Bioaugmentation , Biostimulation , Inorganic nutrient ions , Bacillus subtilis , Crude petroleum , Research Subject Categories::NATURAL SCIENCES::Biology::Organism biology::Microbiology
Citation
Obidi, O.F., Alo, M.N., Nwachukwu, S.C.U. and Aboaba, O.O. (2016). Inorganic Nutrient Ion (PO43-, NO-3 and SO42-) as Essential Requirements in Bioremediation of soils polluted with Crude Petroleum. Nigerian Journal of Microbiology, Vol.30(2), 3409-3414pp.