Antibiotic use in food animals: determination of enrofloxacin residue in chicken tissue
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Date
2017
Authors
AMAGON, K.I
OLAYEMI, S.O
Akinleye, M.O.
AWODELE, O
SILVA, B.O
Journal Title
Journal ISSN
Volume Title
Publisher
West African Postgraduate College of Pharmacists
Abstract
Background: Poultry farmers in Nigeria employ the use of various antibiotics with or without the guidance of
veterinarians, to promote growth and prevent infections in poultry. Although antibiotics benefit most of its
uses, this has led to the accumulation of toxic antibiotic residues in edible poultry products destined for
human consumption.
Objective: The present study was aimed at determining the residual amounts of enrofloxacin in chicken
muscle samples.
Method: Forty birds were obtained from commercial poultries in Lagos State and randomly allotted into 7
groups. They were humanely sacrificed, dissected and 10.0 g of muscle tissue was sectioned from each
drumstick and homogenized in a porcelain mortar. 5.0 g of the homogenate was weighed and transferred into
a 5 ml plain sample bottle and processed for analysis by high performance liquid chromatography to
determine the amount of enrofloxacin in the tissues.
Results: Enrofloxacin was detected in all samples from the four farms. The mean concentration of
enrofloxacin ranged from 23.5 to 88.1 μg/g in all the 7 samples analyzed; these values were higher than the
maximum residue level of 0.1 ìg/ug in Europe and 0.3 ìg/ug in the United States of America.
Conclusion: This study confirmed misuse of enrofloxacin in poultry farms and emphasizes the need for stricter
regulation regarding antibiotic use in poultry as well as the screening of chicken for residues before sale.
Description
Keywords
Enrofloxacin , HPLC , Maxumum Residue Limit , Poultry , Residue
Citation
Kennedy I. Amagon, 1Sunday O. Olayemi, Moshood Akinleye, Olufunsho Awodele and Bola O. Silva (2017) Antibiotic use in food animals: determination of enrofloxacin residue in chicken tissue West African Journal of Pharmacy (2017) 28 (1) 98-106