Effect of Malachite green Concentration on heamatological Parameters of the African catfish (Clarias gariepinus). (Bulletin of Animal Health and Production in Africa
African cat fish (Clarias gariepinus) was exposed to 0.0, 0.10, 0.50, 1.00, 2.0, and 4.5 mg/l concentration of malachite green. The lethal concentration LC5O value of malachite green was 0.38mg/l for 96h of exposure. The effect of sub lethal concentrations of malachite green on blood composition of the Clarias gariepinus (Burchell) was also investigated under semi-static bioassays during 96h exposure. The effects of this chemical on the following fish blood parameters, mean corpuscular value (MCV), mean corpuscular heamoglobin (MCHC), packed cell volume (PCV) heamoglobin (Hb), red blood cell (RBC) and white blood cell (WBC were recorded on the sub lethal concentration of 0.19mg/L at 96h exposure. There were decreases in HB, RBC, PCV and Wbc in the fish showed at sub lethal concentration. Malachite green showed significant increase in MCV, MCH, and MCHC at 96h sub lethal exposure. The changes in heamatological examinations showed that fish treated with malachite green at sub lethal level are faced with a serious metabolic crisis and shifts to alternative methods of metabolism to overcome the toxic stress and maintain its survival in the polluted environment.