Effect of probiotics (Lactobacillus and Bifidobacterium) on Growth performance and Heamatological Profile of Clarias gariepinus.

Ayoola, S. O. ; Ajani, E.K ; Fashae, O.F. (3013-05-01)

Staff publications


This study was carried out to evaluate the use of probiotic (a mixture of Lactobacillus and bifidobacterium species) on growth performance and hematological parameters of Clarias gariepinus juveniles. Fifteen tanks were used and 10 Clarias gariepinus juveniles (mean weight (14.9±0.83 g) per tank, each in triplicate. Five treatment tanks were fed a diet containing 40% crude protein supplemented with varying inclusion of probiotic comprising about 109 colony-forming units per gram of diet (the probiotic diet). Diet T contain 0g probiotic (control diet) while the other groups contain 0.5 g, 1.0 g, 1.5 g and 2.0 g probiotic 0 diet. Results showed that Fish fed with diet T (0.5 g probiotic) had the best growth performance. Weight gain 1 by Fish fed control diet and diet containing 0.5g of probiotic were not significantly different (P>0.05). The final body weight, percentage, weight gain and specific growth rate was positively affected at lowest inclusion of probiotic. Feed conversion ratio of all the treatments were not significantly different (P>0.05). Results showed that there were an increase in the blood parameters of Clarias gariepinus fed probiotic diet treatment compare to the control diet but were not significantly different (P>0.05) at the 30 day. There was significant difference (P<0.05) among the treated groups at day 60. The 90 day of study showed that values of all hematological parameter of fish fed different concentrations of probiotic diet did not differ from the values of fish fed the control diet but there was an increase in platelet value of fish fed control diet which ranges from Packed Cell Volume (16.4-26.8%), Hemoglobin content (4.06-9.23gd LG1), Red blood cells (0.88-2.95×106μ LG1)), White blood cells (171.0-213.μ LG1) and platelets (77.83-322×103μ LG1). There were no significant differences (P>0.05) in the Mean Corpuscular Volume, Mean Corpuscular Hemoglobin and Mean Corpuscular Hemoglobin Concentration, of fish fed different concentrations of probiotic. All blood parameters obtained were between the range of recommended fish blood. It is concluded that using probiotic (especially at 0.5 g) as supplementary feed on Clarias gariepinus showed a slight increase in the hematological parameters compared with the control diet but it has no negative impact on the health status of the studied species. However, probiotic (Lactobacillus and bifidobacterium) can be used as a probiotic agent in aquaculture, to enhance fish health, survival and growth performance.