Detection, inhibition and molecular analysis of multidrug resistant aerobic Gram-negative clinical isolates from a tertiary hospital in Nigeria
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Date
2018-01
Authors
Adeluola, A.O.
Oyedeji, K.S.
Mendie, U.E.
Johnson, J.R.
Porter, J.R.
Journal Title
Journal ISSN
Volume Title
Publisher
Bioline International, African Journals online (AJOL)
Abstract
The challenge of combating the ever emerging multi-drug resistant (MDR) clinical isolates in the face of a slow rate of discovery of new classes of antibiotics is a problem in antibiotic chemotherapy. This study was aimed at (i) linking phenotypic antibiotic drug-resistance characteristics detected in randomly-sampled clinical isolates with detectable genetic markers. (ii) screening a suspected efflux pump inhibitor (EPI) [1-(3-(trifluoromethyl)benzyl]-piperazine (TFMBP)], which could be helpful in combating this challenge. Fifty-one isolates; 28 Klebsiella pneumonia, 3 E. Coli, 1 Enterobacter cloacae, 1 E. aerogenes, 5 Proteus mirabilis, 4 Providencia rettgeri, 1 P. stuartii, 1 Serratia liquefaciens, 6 S. odorifera, and 1 Acinetobacter baumannii obtained from infections of urinary tract, upper respiratory tract, gastrointestinal tract, ear swab, eye swab, and blood culture were screened for (i) antibiotic-susceptibility over a range of 11 classes of antibiotics, (ii) β-lactamase production, (iii) ESBL production and (iv) Efflux pump activity (EPA) in the presence and absence of 1-[3-(trifluoromethyl) benzyl]-piperazine (TFMBP). Molecular analysis was done using DNA extraction by boiling and the randomly-amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) procedure with 2% agarose gel electrophoresis stained with ethidium bromide at 10 µg/ml and visualized by UV trans-illumination. AmpC β-lactamase (4%) and K1 β-lactamase (5.8%) were detected with no carbapenemase producers. AcrA and AcrB marker genes were detected in 12% of the isolates while blaCTX-M (8%) and blaTEM (4%) were also detected. Antibiotic resistance due to EPA can be combated with a suitable EPI as demonstrated by TFMBP when combined with specific antibiotics.
Description
Staff publications
Keywords
TFMBP , Efflux pump activity , ESBL , MDR , Carbapenemase , Research Subject Categories::PHARMACY::Pharmaceutics
Citation
Adeluola, A. O., Oyedeji, K. S., Mendie, U. E., Johnson, J. R., & Porter, J. R. (2018). Detection, inhibition and molecular analysis of multidrug resistant aerobic Gram-negative clinical isolates from a tertiary hospital in Nigeria. African Journal of Biomedical Research, 21(1), 15-21.