In-vitro Analytical techniques for PCBs & OCPs in Plasma Employing Solid-phase Disk Extraction Technique.
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Date
2018
Authors
Adeyemi David, Adeoye Oyerinde, Johnson Oluwatosin and Adeleye Adedayo
Journal Title
Journal ISSN
Volume Title
Publisher
Nig. J. Pure & Appl. Sci.
Abstract
Solid phase extraction techniques are generally simple, economical and use less solvent, making them more appropriate for use in epidemiological studies. The Current study was focused on application of C-18 solid phase extraction coupled to sampli Q Alumina B cartridges in isolation of 7 polychlorinated biphenyls and 16 organochlorine pesticides from plasma samples donated by volunteers from population in Lagos Nigeria, followed by quantification with GC-ECD. Mean recoveries (n=4) vary from 90-92 % with a good reproducibility (RSD ≤ 8 %), while LOD range from 0.01-0.05 mg/L. Linearity range of calibration curves were 0.02-2.00 and 0.10-2.0 mg/L for PCBs and OCPs respectively while correlation coefficients (R2) ranged from 0.997-0.999. Mean concentrations (mg/L) of PCBs and OCPs in samples were 0.0849-1.4785 and 0.3781-1.2473 respectively, while for low and high chlorinated PCBs were 0.0849-1.374 and ND-0.1709 respectively. Also, the mean concentrations (mg/L) of total DDTs and BHCs ranged from 0.02796-0.96491 and 0.01051- 0.05751 respectively. The most frequent detected were PCB 28 and permethrin, which were detected in all samples, while the least detected were PCB 180 and heptachlor epoxide, which were detected in 20 and 50% of samples respectively. The study shows that the analytical method gave good recoveries for the analytes, while the mean concentrations of OCPs were relatively higher than PCBs in 90% of samples investigated. The PCA result shows that the PCBs and OCPs were present in similar range in all the samples/variables investigated. The variables components for PCA 1 positive loading (PCB 52) remarkably correlated and suggesting that all the samples have similar origin of contamination.
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Keywords
Solid phase extractions, plasma, mean concentrations, recoveries, analytical method, and contamination.