Antioxidant and Hypoglycaemic activities of the Ethanol extract of Senecio Biafrae leaves.
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Date
2017
Authors
G. A. Ayoola, O. O. Johnson, D. K. Adeyemi, O. M. Lapite, C. O. Doherty
Journal Title
Journal ISSN
Volume Title
Publisher
Chemical Society of Nigeria
Abstract
Diabetes mellitus is among the most common disorder in developed and developing countries, it has been characterized by increased levels of oxidative stress and hyperglycaemia which are implicated in the development of diabetic complications. Therefore, the aim of this study is to investigate the antioxidant and hypoglycaemic effect of the ethanol leaf extract of Senecio biafrae Oliv & Hiern Compositae, using DPPH and alpha amylase inhibition methods respectively. S. biafrae is known for its therapeutic virtues, notably in Nigeria where it is used in the treatment of diabetes or pulmonary defects. The plant extract displayed appreciable level of antioxidant activities by standard assay methods: The DPPH (1, 1-diphenyl-2-picryhydrazyl) free radical scavenging assay showed percentage inhibition range from 44.26±0.069% – 49.96±0.075% for Senecio biafrae leaf extract, 95.83±0.023% – 98.88±0.002% for ascorbic acid (standard) and 54.63±0.038% –58.99±0.033% for alpha-tocopherol (standard), with IC 50 136.95 µg/ml, 6.65 µg/ml and 17.14 µg/ml respectively at concentration ranging from 20 to 100 µg/ml. The Ferric reducing antioxidant power assay (FRAP) of the extract was 147.3 μM Fe 2+/g dry extract while the positive control ascorbic acid had a value of 265.4 μMFe 2+/g dry material. The hypoglycaemic activity using α-amylase inhibition assay revealed inhibition from 21.78±0.002% - 74.67±0.0003% for Senecio biafrae leaf extract and 6.6±0.026% – 48.89±0.00% for acarbose (standard drug), with IC50 86.20 µg/ml and 513.5 µg/ml respectively at concentration ranging from 20-500 µg/ml. Therefore, the results indicated that the extract of Senecio biafrae possess significant antidiabetics and in-vitro antioxidant activities.
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Keywords
Hypoglycaemic, antioxidant, α-amylase, Senecio biafrae leaf and phytochemicals