Development and characterization of collagen‑based electrospun scaffolds containing silver sulphadiazine and Aspalathus linearis extract for potential wound healing applications
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Date
2020
Authors
Ilomuanya, M.O.
Adebona, A.C.
Wang, W.
Sowemimo, A.
Eziegbo, C.L.
Silva, B.O.
Adeosun, S.O.
Joubert, E.
Beer, D.D.
Journal Title
Journal ISSN
Volume Title
Publisher
SN Applied Sciences
Abstract
Bone, skin and soft tissue chronic wounds emanating from burns or bacterial infections which persist due to prolonged
tissue inflammation contribute to a delay in wound healing. Electrospun biomimetic scaffolds produced from biodegradable polymers have proven to be a better alternative due to their large surface area to volume ratio and ability to
release the drug directly to the wound surface allowing fast and sustained absorbance over the affected wound area.
In this study, poly lactic acid (PLA) (20% w/v) and collagen-based (PLA/C) fibrous scaffolds (electrospun at a voltage of
22 kV, flow rate of 0.1 mL/min) containing varying concentrations of silver sulphadiazine (1% w/w, 0.75% w/w) (Ag+S)
and Aspalathus linearis (AL) fermented extract (0.025%, 0.1% and 0.5% w/w), were designed and fabricated to increase
antimicrobial penetration and cellular biocompatibility. The elastic modulus of samples revealed that incorporating 1%
Ag+S and A. linearis extract to PLA solution culminates in a fiber with the superlative stiffness of 2.1.1 GPa. The antimicrobial effect of the scaffolds was evaluated against S. aureus, P. aeruginosa, MRSA and E. coli. PLA/C–Ag+S/AL scaffolds and
showed antibacterial activity against both gram +ve and gram −ve bacteria. They were nontoxic to the cells and provided
favorable substrates for the neonatal epidermal keratinocytes cells to undergo cell attachment and proliferation. PLA/C–
Ag+S/AL scaffolds have a great potential for use in chronic wounds as well as in tissue and bioengineering applications.
Description
Keywords
Electrospun nanofibers · Silver sulphadiazine · Wound healing · Aspalathus linearis · Poly lactic acid
Citation
Ilomuanya, M.O. Adebona, A.C. Wang, W. Sowemimo, A. Eziegbo, C.L. Silva, B.O. Adeosun, S.O. Joubert, E. Beer, D.D. (2020). Development and characterization of collagen‑based electrospun scaffolds containing silver sulphadiazine and Aspalathus linearis extract for potential wound healing applications. SN Applied Sciences; 2:881