Antioxidant, antinociceptive and anti-inflammatory properties of the aqueous and ethanolic leaf extracts of Andrographis paniculata in some laboratory animals.
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Background: The study was designed to evaluate the anti-inflammatory, analgesic and antioxidant properties of Andrographis paniculata leaf extracts in laboratory animals. Methods: The dried and powdered leaves of the plant were subjected to phytochemical and proximate analy-ses. Its mineral content was also determined. Acute toxicity experiments were first performed to determine a safe dose level. The plant material was extracted using water and ethanol as solvents. These extracts were then used to test for the anti-inflammatory, analgesic and antioxi-dant properties of the plant. The anti-inflammatory tests included carrageenan-induced and histamine-induced paw oedema. The analgesic tests conducted were formalin paw lick test and acetic acid writhing test. The antioxidant activities of the extracts of A. paniculata were deter-mined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), total polyphenol (TP) and 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) using ascorbic acid as standard for both DPPH and FRAP, and gallic acid as a standard for both TP and ABTS. Results: The acute toxicity experiment demonstrated that the plant is safe at high doses even at 1600 mg/kg. It was observed that the ethanolic extract of A. paniculata had higher antioxidant activity than the aqueous extract. The experiments using both extracts may suggest that the extracts of A. paniculata leaves possess anti-inflamma-tory, analgesic and antioxidant properties, although the ethanolic extract seemed to have higher biological prop-erties than the aqueous extract. Conclusions: The results from this study may have justi-fied the plant’s folkloric use for medicinal purpose.
Ethanolic extract , Andrographis paniculata , Anti-inflammatory , Phytochemical , Research Subject Categories::PHARMACY::Pharmacognosy
Adeolu Alex Adedapo, Bisi Olajumoke Adeoye, Margaret Oluwatoyin Sofidiya, Ademola Adetokunbo Oyagbemi (2015). Antioxidant, antinociceptive and anti-inflammatory properties of the aqueous and ethanolic leaf ext Andrographis paniculata in some laboratory animals. Journal of Basic Clinical Physiology and Pharmacology 26 (4), 327-334.